Preparation and evaluation of monolithic molecularly imprinted stationary phase for S-naproxen
De-Miao Chen1,2,Qiang Fu1,Wei Du1,Si-Juan Sun1,Ping Huang1,Chun Chang11 Xi'an Institute for Food and Drug Control,Xi'an 710054,China;2 Faculty of Pharmacy,School of Medicine,Xi'an Jiaotong University,Xi'an 710061,China.
An S-naproxen(S-NAP)molecularly imprinted monolithic stationary phase(MIMSP)with specific recognition for S-NAP and naproxen(NAP)was prepared by in situ technique,utilizing 4-vinylpridine(4-VP)as a function monomer,ethylene glycol dimethacrylate(EDMA)as a cross-linking agent,and low-polar solvents(toluene and dodecanol)as porogenic solvents.The selectivity of the polymers for S-NAP and NAP was evaluated by high performance liquid chromatography(HPLC).The binding characteristics were tested by Scatchard analysis.Racemic NAP could be specifically separated to some extent.At the same time,NAP could be separated from ibuprofen under optimized conditions.Scatchard analysis showed that two classes of binding sites existed in the S-NAP-imprinted polymers,with their dissociation constants estimated to be 1.045 and 5.496 μM,respectively.The results demonstrate that S-NAP and NAP can be recognized specifically on the obtained MIMSP.
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| Chinese Journal Full-text Database |
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LIU Zhao-sheng~1 SU Jing~1 ZHENG Chao~2 HAO Lan-fang~1 GAO Ru-yu~2 (1.College of Pharmacy,Tianjin Medical University,Tianjin 300070,China;2.Institute of Element Organic Chemistry,Nankai University,Tianjin 300071,China);Application of molecular imprinted monolith in drug separation[J];Chinese Journal of Pharmaceutical Analysis;2006-11 |
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LI Yi-rui,ZHANG Min-Ru*(Shanghai Institute of Pharmaceutical Industry,Shanghai 200040);Progress of Molecularly Imprinted Monolithic Column for HPLC[J];Chinese Journal of Pharmaceuticals;2007-04 |
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| China Proceedings of conference Full-text Database |
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