Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

Protective effect of allicin against oxidative stress and hepatocyte autophagy in iron-overloaded rats

LIU Chong-bin1,WANG Rui1,DONG Miao-wu2,WU Bo2,XIAO Min2(1.Department of Physiology and Pharmacology,School of Medicine,Huzhou Normal College,Huzhou 313000,China;2.Center of Animals,Wenzhou Medical College,Wenzhou 325035,China)  
OBJECTIVE To determine the inhibitory effect of allicin on oxidative stress,and hepatocyte autophagy and defence mechanisms.METHODS Thirty-six SD rats were randomly divided into normal(basal diet,Fe 50 mg·kg-1),iron model(high iron diet,Fe 1000 mg·kg-1),normal+allicin 40 mg·kg-1(basal diet,ig given allicin),iron model+allicin 30,40 and 60 mg·kg-1(high iron diet,ig given allicin) groups.These rats were fed for 6 weeks.Serum and tissue(the liver,colon and kidneys) iron concentrations,total iron-binding capacity(TIBC),the level of malondialdehyde(MDA) and total superoxide dismutase(T-SOD) were quantified by reagent kit method.At the same time,the morphological changes of the liver,hepatocyte autophagy and caspase 3,Ki-67 and LC3-B protein expression were evaluated through ultrastructural observation and immunohistochemistry.RESULTS Compared with normal group,the serum iron concentration,TIBC and the liver and colon iron concentrations increased significantly in the iron model group(P0.05,P0.01).Compared with the iron model group,serum and colon iron concentrations decreased(P0.05) and TIBC increased(P0.05) in iron model + allicin groups;liver iron concentrations decreased significantly(P0.05) in iron model+allicin 40 and 60 mg·kg-1 groups.Compared with normal group,the level of MDA in liver and colon and T-SOD activities in the liver increased(P0.05,P0.01) while T-SOD activities in the colon decreased significantly(P0.01).Compared with iron model group,the level of MDA in the liver decreased significantly(P0.05) while T-SOD activities in the colon and kidneys increased significantly(P0.05).Compared with normal group,cell proliferation increased significantly in hepatocytes and in non-parenchymal cells induced by iron supplementation in the iron model group(P0.05),but caspase 3 immunostaining had no significant difference between these groups.In addition,iron was accumulated within the hepatic lysosomes where it triggered autophagy as evidenced by the formation of autophagic vesicles in the iron model group.It also induced morphologic alterations of the mitochondrial and endocytoplasmic reticulum membranes.In addition,LC3-B was strongly expressed in the iron model group,but was not expressed or attenuated in the normal and normal+allicin groups.CONCLUSION Allicin can scavenge free radicals efficiently,enhance antioxidant properties,depress the iron-induced oxidation pressure and morphologic alterations of the mitochondrial membranes and cell autophagy.Allicin has potent protection effect on hepatocytes in chronic iron intoxication.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved