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《Chinese Journal of Difficult and Complicated Cases》 2008-09
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Effect of Qingdushuan on apoptosis of cervical cancer SiHa cell

YU Yan-yan,LOU Jiao-ying,JIN Zhe.Beijing Traditional Chinese Medicine University,Beijing 100076,China  
Objective To study proliferation and apoptosis of cervical cancer treated by Qingdushuan in cell biology and molecular biology.Methods 4 groups(blank serum group,Qingdushuan group,Baofukang group and Andafen group)were developed,and the proliferation inhibiting effect of the drugs on AiHa cells(cultivated with 4%,containing serum)were detected by MTT assay.At the same time,apoptosis was detected using flow cytometry and TUNEL method.Results After cultured with medium containing drug,SiHa cells were significantly decreased,of which at 4 % concentration of serum and after 72 h was most.Compared with the blank serum group,inhibiting effect of the 3 drug treated groups were significant high(P0.01).4% concentrations of serum and at 72 h have a marked impact on cell apoptosis.Conclusion Qingdushuan containing serum inhibited tumor through the promotion of cell apoptosis.
【Fund】: 国家自然科学基金青年项目(No.30600831)
【CateGory Index】: R737.33;R285.5
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