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《Chinese Journal of Difficult and Complicated Cases》 2009-01
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The effect of mobilizing autologous bone marrow stem cells on the expression of bFGF in rats after cerebral ischemia/reperfusion

GE Zhao-li,BAI Run-tao,HAN Man-fu.Department of Neurology, Shenzhen Secondary People's Hospital,Shenzhen 518035,China  
Objective To investigate the expression of basic fibroblast growth factor (bFGF) in mobilizing autologous bone marrow stem cells on focal cerebral ischemia/reperfusion in rats. Methods Sixty rats were divided randomly into the granulocyte colony stimulating factor (G-CSF) treatment group and control group. Both groups were divided again into 7 d, 14 d and 21 d subgroups according to the different time point. The MCAO/R model was induced with filament occlusion method, The treatment group was administrated G-CSF once a day for 5 days per 50μg/kg with hypodermic injection after model built. Yet the control group was applied with sodium chloride. The neurological severity scores (NSS) were evaluated in different time points. Immunohistochemistry technique was used to detect positive cells of bFGF. Results After MCAO/R model built, the NSS of G-CSF group in 7 d, 14d and 21d was 4.0±0.9,3.8±1.1,3.5±1.3 respectively,the control group was 5.5±1.3, 5.8±1.4 and 5.7±1.6 respectively. The NSS in treatment group was lower than the control group (P0.05). The positive cells of bFGF were detected in both G-CSF treatment group and control group, which situated in the marginal zone of the cerebral infarction, and gradually decreased along with time extends. The quantitative (per HP) statistical of bFGF positive cells in the G-CSF treatment group was 25.4±4.2, 17.1±3.3 and 12.6±3.4 respectively,and the control group was 18.0±4.0, 12.0±3.1 and 6.4±2.0. It showed that the positive bFGF cells in treatment group was higher than control group (P0.05).Conclusion It suggested that G-CSF could improve neural function and increase the expression of bFGF in marginal zone of the cerebral infarction after cerebral ischemia/reperfusion in rats.
【CateGory Index】: R743.3
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