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《Chinese Journal of Difficult and Complicated Cases》 2010-12
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Experimental study of antiproliferative effect of t-PA combined with MMC in glaucoma filtration surgery

REN Yan-xin,MA Jing-xue,WEI Yu-cai,et al.Department of Ophthalmology,the Second Hospital of Hebei Medical University,Shijiazhuang 050011,China  
Objective To investigate the antiproliferative effect of t-PA combined with MMC in filtration surgery. Methods Forty New Zealand white rabbits were randomly divided into four groups and standard trabeculectomy was processed to every rabbit eyes.Then,a piece of cotton with 0.2 mg/ml MMC was placed under sclera flap for 5 minutes(group B),0.1ml t-PA(250μg/ml) was injected into the anterior chamber at the end of operation and 0.3ml t-PA(250μg/ml) was injected into bulbar conjunctiva on the lst、3rd day after operation(group C),a piece of cotton with 0.1 mg/ml MMC was placed for 5 minutes and 0.1ml t-PA(200μg/ml) was injected into the anterior chamber at the end of operation followed with 0.3ml(200μg/ml t-PA) conjunctival injection on the 1st、3rd day after operation(group D).On days 3,7,14 and 28 postoperatively,the filtration passage was observed by HE staining, the collagen formation was observed by masson trichrome staining.After performing immunohistochemical staining, the expression of proliferative cell nuclear antigen(PCNA) in the filtration area was observed and the number of positive cell nuclear was counted by microscope.Results All filtration passages were opened on 3rd day after surgery. The percentage of opened filtration passage was higher in group D than in the other three experimental groups after 7, 14,28 days.There was no collagen in the wound area on the 3rd day after surgery in four groups.But on the 7th day after surgery,a little collagen was seen in the group A.On the 14th day after surgery,dense collagen appeared in group A,while only a little collagen was observed in the other three groups.After 28 days of operation,still a little collagen was observed in experiment group D,and the expression was weaker than the in group A,B and C(P0.05).Conclusion Trabeculectomy with lower dose mitomycin C combined with t-PA was effective on antiproliferation in glaucoma filtration surgery.
【Fund】: 河北省科技攻关计划项目(No.052761227)
【CateGory Index】: R779.6
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