Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Difficult and Complicated Cases》 2013-01
Add to Favorite Get Latest Update

The influences of Qiweikeli on the expression of USF2 and renal fibrosis in KK-Ay mice with diabetic nephropathy

MA Ming-fei~*,GAO Yan-bin,WANG Jin-yang,et al.* Dongfang Hospital,Beijing Traditional Chinese Medicine University, Beijing 100078,China  
Objective To investigate the effects of Qiweikeli on the expression of USF2 and renal fibrosis in KK-Ay mice with spontaneous type 2 diabetic nephropathy.Methods 60 SPF grade 12-week old male KK-Ay mice were randomly divided into three groups,model group,Qiweikeli group,valsartan group,normal control was 20 C57BL/6J mice.With Real time PCR and Western blot detection of renal tissue USF2,by its regulation induced fibrosis factor gene and protein expression of TGF-β_1,and regulation downstream fibrosis protein gene and protein expression.24 weeks of age,blood glucose,glycosylated hemoglobin,renal function(BUN,SCr),urinary protein biochemical parameters were detected;renal structural changes with light microscopy and electron microscopy,renal fibrosis with Masson staining were used for analysis.Results(1)Compared with model group,USF2 and TGF-β_1 mRNA and protein were significantly higher in renal tissue of the model group,the expression of TGF-β_1,Col-Ⅳ,FN increased than control group,the degree of fibrosis was significantly severe,renal function decreased obviously(P 0.05).(2)Compared with the model group,renal function and urinary protein were improved in Qiweikeli group and valsartan group,renal fibrosis was released,USF2 and TGF-β_1 gene and fibrosis protein levels were significantly lower(P 0.05).(3)Correlation analysis indicate that USF2 was positively correlated to TGF-β_1(r=0.82,P 0.05).Conclusion It provided that the Qiweikeli could reduce the expression USF2 gene and protein in the spontaneous type 2 diabetic KK-Ay mice kidney tissue,this may be one of the mechanisms for its treatment of DN by regulating signal transduction.
【Fund】: 北京市教育委员会科技计划重点项目(No.KZ201110025025);; 国家自然科学基金项目(No.81173238);; 国家重点基础研究发展计划(973计划)项目(No.2012CB518602)
【CateGory Index】: R285.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 MA Ming-fei1,2,GAO Yan-bin2,WANG Jin-yang2,3,ZHANG Na2,ZHOU Guang-chun4,YAO Jing-juan2,ZHAO Xuan2,ZHU Zhi-yao2,ZOU Da-wei2(1Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China;2Department of Endocrine and Metabolism,Capital Medical University of Traditional Chinese Medical School,Beijing 100069,China;3Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China;4Tongzhou Orthopedics Hospital of Integrated Tranditional and Western Medicine,Beijing 101100,China);Influences of Qi-wei Granules on USF2 and its downstream signal transduction in KK-Ay mice with diabetic nephropathy[J];China Journal of Traditional Chinese Medicine and Pharmacy;2013-05
China Proceedings of conference Full-text Database 1 Hits
1 MA Ming-fei~(1,2),GAO Yan-bin~2,WANG Jin-yang~(2,3),ZHANG Na~2,ZHOU Guang-chun~4,YAO Jing-juan~2, ZHAO Xuan~2,ZHU Zhi-yao~2,ZOU Da-wei~2 (1 Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China;2 Department of Endocrine and Metabolism, Capital Medical University of Traditional Chinese Medical School,Beijing 100069,China;3 Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China;4 Tongzhou Orthopedics Hospital of Integrated Tranditional and Western Medicine, Beijing 101100,China);Influences of Qi-wei Granules on USF2 and its downstream signal transduction in KK-Ay mice with diabetic nephropathy[A];[C];2013
【Citations】
Chinese Journal Full-text Database 2 Hits
1 LI Ying; WU Wenqing;ZHANG Yimin, et al (Department of Internal Medicine, the Third Hospital of Hebei Medical University,Shijiazhuang 050051, China);The effect of erigeron breviscapus on renal protein kinase C in diabetic rats[J];CHINESE JOURNAL OF NEPHROLOGY;2000-02
2 LIU Zhihong; LI Yingjian; ZHANC Jing; et al.(Institute of Nephrology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002);Modulatory effect of transforming growth factor-beta and Rhein on glucose transporter-1 in human glomerular mesangial cells[J];NATIONAL MEDICAL JOURNAL OF CHINA;1999-10
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Anhui Medical College ,Hefei 230061 GUO Yan,CHEN Xi;Observation of curative effect of astragalus membranaceus injection together with hypoglycemic agent on diabetic microalbuminuria[J];Journal of Anhui Health Vocational & Technical College;2004-02
2 Anhui Provincial Hospital,Heifei 230001,Anhui DONG Juan;Experience of nursing on one case of medullany spongekidney with multiple kidney stones[J];Journal of Anhui Health Vocational & Technical College;2008-01
3 ZHANG Xiang-ru Department of Nephrology, Luan People's Hospital,Luan237005,China;199 cases of pathological analysis of adult glomerular diseases[J];Journal of Anhui Health Vocational & Technical College;2008-02
4 The Lu'an People's Hospital,Lu'an 237005,Anhui BAI You-wei;Clinical Trial on 53 IgA Nephropathy Patients[J];Journal of Anhui Health Vocational & Technical College;2009-01
5 Zhang Lin,Gao Bin,Hu Shurong,et al\;Department of Ridneymedical,Hefei First Pecople's Hospital,Hefei 230061\;;The comparison of kidney biopsy guided by CT and B-US[J];Anhui Medical Journal;2001-06
6 Wang Suhang, Zhu Jiang, Zong Weijun, et alDepartment of Internal Medicine, Huaibei People's Hospital, 235000;Clinical effect of losartan combined with captopril in patients with diabetic nephropathy[J];;2006-01
7 Xu Jialing, Lian Juan, Liu Ping, et al Department of Nephrology, Department of Ultrasound, the 105th of PLA, Hefei 230031;Analysis of success rate and safety factor of ultrasound-guided biopsy of renal tissue[J];Anhui Medical Journal;2008-05
8 Li Huai,Hao Li,He Hengjie,et al Department of Nephrology,the Second Hospital of Anhui Medical University,Hefei,230601,China;The application of using easy peritoneal dialysis to rescue critical patients with renal failure[J];Anhui Medical Journal;2010-03
9 Zhang Xiangwen,Xia Jingbiao,Xu Shuangshuang,et al Department of Nephrology,the First College of Clinical Medical Science,China Three Gorges University,Yichang 443003,China;Comparison of different way of renal biopsy under ultrasound guide[J];Anhui Medical Journal;2011-09
10 QIAN Guang Rong, JIAO Bao Zhu (Dept of Nephrology, Maanshan People's Hospital, Maanshan 243000);Comparison between central venous catheter (CVC) and atrerio-puncture (AP) in hemodialysis[J];Anhui Medical and Pharmaceutical Journal;2003-05
China Proceedings of conference Full-text Database 9 Hits
1 Zhang Shi-wen(Ganzhou TCM Hospital of Jingxi Province,Ganzhou 341000,Jiangxi,China);WuJianXin traditional Chinese medicine treatment on chronic nephritis clinical experience[A];[C];2011
2 (Analysis Department of The third people hospital in Hangzhou,Hangzhou in Zhejiang Province,310009) Chen Song,Yang Yonggang;Correlation analysis of Urine electric conductivity and kidney far tubule function[A];[C];2011
3 WANG Yuefei, XU Ping, YANG Xianqiang (Department of Tea Science, Zhejiang University, Hangzhou 310029);Clinical Trial of Tea Polyphenols Tablet on Chronic Glomerulonephritis[A];[C];2008
4 ;The Clinical Study of The treatment on the Yi shen bu xue san in Renal Anemia[A];[C];2009
5 MA Ming-fei~(1,2),GAO Yan-bin~2,WANG Jin-yang~(2,3),ZHANG Na~2,ZHOU Guang-chun~4,YAO Jing-juan~2, ZHAO Xuan~2,ZHU Zhi-yao~2,ZOU Da-wei~2 (1 Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China;2 Department of Endocrine and Metabolism, Capital Medical University of Traditional Chinese Medical School,Beijing 100069,China;3 Beijing Tongren Hospital,Capital Medical University,Beijing 100730,China;4 Tongzhou Orthopedics Hospital of Integrated Tranditional and Western Medicine, Beijing 101100,China);Influences of Qi-wei Granules on USF2 and its downstream signal transduction in KK-Ay mice with diabetic nephropathy[A];[C];2013
6 Zhangshengyu Shitianlu Jianglin (Anhui Provincial Hospital of Medical University Pharmacy anhui hefei 230001);Clinical pharmacists involved in CKD with Exfoliative dermatitis caused by allopurinol patients program design and pharmaceutical care[A];[C];2013
7 Li Guohua,Li Yudan,Ding Zhisheng,Cai Xue,Zhang Zhiwen (Nanchang Traditional Chinese and Western Medicine Combining Hospital Nanchang,Jiangxi 330003);The clinical investigation of the treatment of tonifying kidney and resolving dampness particle plus irbesartan for diabetic nephropathy[A];[C];2013
8 ;On the TCM treatment of renal edema[A];[C];2012
9 LIN Lan;GUO Xiao-zhou;NI Qing;ZHAO Hao;GONG Yan-bing;WANG Bin;LIU Ying;Guang'anmen Hospital,China Academy of Medical Sciences;Daxing Hospital of Tradtional Chinese Medicine;;Risk Factors and TCM Patterns of Early Diabetic Nephropathy[A];[C];2012
【Co-references】
Chinese Journal Full-text Database 2 Hits
1 LI Ying; WU Wenqing;ZHANG Yimin, et al (Department of Internal Medicine, the Third Hospital of Hebei Medical University,Shijiazhuang 050051, China);The effect of erigeron breviscapus on renal protein kinase C in diabetic rats[J];CHINESE JOURNAL OF NEPHROLOGY;2000-02
2 LIU Zhihong; LI Yingjian; ZHANC Jing; et al.(Institute of Nephrology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002);Modulatory effect of transforming growth factor-beta and Rhein on glucose transporter-1 in human glomerular mesangial cells[J];NATIONAL MEDICAL JOURNAL OF CHINA;1999-10
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 LI Guang-chao;CHE Nian-cong;GENG Jian-guo;ZHAO Hui;WANG Zhi-qiang;GUO Xin-xin;BAI Chen;College of TCM,Capital Medical University;Pinggu Hospital of TCM;;Discussion of changes of TGF-β1,Smad-4,α-SMA and PDGF in DN rat liver before and after treatment in traditional Chinese Medicine based on the “liver and kidney share the same origin”[J];Chinese Journal of Basic Medicine in Traditional Chinese Medicine;2014-04
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 Xu Guang, Zhang li- Ping, Shen Hui-Fen, Hu Chang-Qi(Department of Biochemistry, School of Basic Medical Sciences, Department of Chemistry of Natural Drugs, School of Pharmacy, Shanghai Medical University, Shanghai);INHIBITION OF PROTEIN KINASE C BY SCUTELLAREIN AND ITS ANALOGUES[J];;1993-03
2 Zhang Jing,Liu Zhihong,Chen Zhaohong,Liu Dong,Li Leishi Research Institute of Nephrology, Jinling Hospital (Nanjing, 210002);mRNA EXPRESSION AND FUNCTION OF GLUCOSE TRANSPORTER 1 IN MOUSE GLOMERULAR MESANGIAL CELLS[J];CHINESE JOURNAL OF NEPHROLOGY, DIALYSIS & TRANSPLANTATION;1998-03
3 Yang Jun-wei, et al. Jingling Hospital.Nanjing 210002;EFFECT OF RHEUM OFFICINALE ON EXPERIMENTAL DIABETIC NEPHROPATHY[J];Chinese Journal of Endocrinology and Metabolism;1993-04
4 Yang Jun-wei,Li lei-shi, Liu Zhi-hong,et al.Institute of Nephrology,Jinling Hospital,Nanjing University,210002;RHEUM OFFICINALE DOWN REGULATEDGENE EXPRESSION OF GROWTH FACTORS IN GLOMERULI FROM DIABETIC RATS.[J];CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM;1995-04
5 Chen Li -meng,Li Xue-wang,Huang Li -wei, Li Yan, Duan Lin, Zhang Xiao-juan (Department of Nephrology,PUMC Hospital,CAMS and PUMC,Beijing100730,China);The Early Pathological Changes of KKA~y Mice with Type2 Diabetes[J];Acta Academiae Medicinae Sinicae;2002-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved