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《Chinese Journal of Difficult and Complicated Cases》 2013-01
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The influences of Qiweikeli on the expression of USF2 and renal fibrosis in KK-Ay mice with diabetic nephropathy

MA Ming-fei~*,GAO Yan-bin,WANG Jin-yang,et al.* Dongfang Hospital,Beijing Traditional Chinese Medicine University, Beijing 100078,China  
Objective To investigate the effects of Qiweikeli on the expression of USF2 and renal fibrosis in KK-Ay mice with spontaneous type 2 diabetic nephropathy.Methods 60 SPF grade 12-week old male KK-Ay mice were randomly divided into three groups,model group,Qiweikeli group,valsartan group,normal control was 20 C57BL/6J mice.With Real time PCR and Western blot detection of renal tissue USF2,by its regulation induced fibrosis factor gene and protein expression of TGF-β_1,and regulation downstream fibrosis protein gene and protein expression.24 weeks of age,blood glucose,glycosylated hemoglobin,renal function(BUN,SCr),urinary protein biochemical parameters were detected;renal structural changes with light microscopy and electron microscopy,renal fibrosis with Masson staining were used for analysis.Results(1)Compared with model group,USF2 and TGF-β_1 mRNA and protein were significantly higher in renal tissue of the model group,the expression of TGF-β_1,Col-Ⅳ,FN increased than control group,the degree of fibrosis was significantly severe,renal function decreased obviously(P 0.05).(2)Compared with the model group,renal function and urinary protein were improved in Qiweikeli group and valsartan group,renal fibrosis was released,USF2 and TGF-β_1 gene and fibrosis protein levels were significantly lower(P 0.05).(3)Correlation analysis indicate that USF2 was positively correlated to TGF-β_1(r=0.82,P 0.05).Conclusion It provided that the Qiweikeli could reduce the expression USF2 gene and protein in the spontaneous type 2 diabetic KK-Ay mice kidney tissue,this may be one of the mechanisms for its treatment of DN by regulating signal transduction.
【Fund】: 北京市教育委员会科技计划重点项目(No.KZ201110025025);; 国家自然科学基金项目(No.81173238);; 国家重点基础研究发展计划(973计划)项目(No.2012CB518602)
【CateGory Index】: R285.5
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