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Changes of blood-brain barrier structure and aquaporin 4 in mice with acute liver failure

JIAO Fangzhou;ZHANG Haiyue;WANG Luwen;GONG Zuojiong;Department of Infectious Diseases,Renmin Hospital of Wuhan University;  
Objective To observe the changes of blood-brain barrier structure and aquaporin 4(AQP4) in brain edema of mice with acute hepatic failure.Methods All male C57BL/6 mice were divided into control and model group.The ALF experimental animal models were induced by a combination of lipopolysaccharide(LPS) and D galactosamine(D Gal).The BBB of integrity was assessed by transmission electron microscope.Liver histologic analysis was performed,the brain protein of AQP4,and levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),serum ammonia were observed.Results The model group had obvious pathological liver tissue damage,but there was no significant change in the control group.Compared with the control group,the serum ALT,AST and serum ammonia levels in the model group increased significantly(t=5.075,t=6.338,t=5.718,P0.05).The model group of brain blood brain barrier structure was abnormal,can be observed by electron microscopy and micro blood vessels around the astrocyte swelling,endothelial cells appeared large vesicles and vacuoles,endothelial cell tight junction opened.The expression level of AQP4 protein in brain tissue was not significantly different from that in control group(t=-0.961,P=0.397).Conclusion There is a slight change in the blood-brain barrier structure in patients with acute hepatic failure with cerebral edema.The mechanism of vascular edema is involved in the brain edema of acute hepatic failure,and the level of AQP4 protein in brain tissue has not changed significantly.
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