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《Acta Botanica Yunnanica》 1985-01
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STUDIES ON MABINLIN, THE SWEET PROTEIN FROM SEEDS OF CAPPARIS MASAIKAI I . COMPARISON OF MABINLIN I TO MABINLIN Ⅱ

Hu Zhong, Peng Liping and He Min (Kunming Institute of Botany, Academia Sinica)  
Two components of sweet protein were saparated and characterized: mabinlin I (MaI), the major one, with MW of 11600 and isoelectric point pI of 11.8, and mabinlin Ⅱ (Ma Ⅱ ) with MW of 10400 and pI of 11.3. Another component mabinlin Ⅲ (Ma Ⅲ) with MW of 10200 and pI of about 11.8 was found to be produced during the extraction. MaⅡ contains 84 amino acid residues that are fewer 15 amino acid residues than Ma I , of which 6 residues are Pro. These proteins are all single polypeptide chains lacking Ser and Met. 80 amino acid residues are common in Ma I and Ma I molecules in which Arg, Glx and Pro are dominant ones. No SH group was detected. After treatment of reductive cleavage withβ -mercaptoethanol in 8 M urea, these protein molecules were transformed into dimers and lost the sweet nature. The yield and the ratio of Ma I to Ma Ⅱ extracted from the lipid-free powder of decorticated seeds depended on the solvent used and the conditions of extraction. A highest yield of 13% was obtained in extraction with 50% aqueous solution of acetone and saparation through CM-cellulose column chromato-graphy. While extraction with watar or 0.lM pH6.2 phosphate buffer the yield was less than 1% because of the less accessibility of the protein in combined state.
【Fund】: 国家科委资助
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【References】
Chinese Journal Full-text Database 2 Hits
1 Ding Ming Hu Zhong (Kunming Institute of Botany, Academia Sinica);STUDIES ON MABINLIN, THE SWEET PROTEIN FROM SEEDS OF CAPPARIS MASAIKAI Ⅳ. STABILITY AND DENATURATION[J];Acta Botanica Yunnanica;1986-02
2 Liu Xinozhu Hu Zhong (Kunming Institute of Botany, Academia Sinica, Kunming);N-AND C-TERMINAL PARTIAL AMINO ACID SEQUENCE DETERMINATION OF THE SWEET PROTEINS FROM THE SEED OF CAPPARIS MASAIKAI[J];Acta Botanica Yunnanica;1988-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 YOU Yin-wei~1, WANG Tian-hong~(1), YUE Shou-song~2(1.State Key Lab of Microbial Technology,Shandong University,Jinan 250100,China;2.Shandong Academy of Agricultural Sciences, Jinan 250100,China);Morphological observations and studies on cellulase propertiesof the oceanic Penicillium sp.FS010441[J];Shandong Agricultural Sciences;2005-01
2 WANG Huan et al (College of Chemical Engineering,Shenyang Institute of Chemical Technology,Shenyang,Liaoning 110142);New Technology of Producing Sugar by Acid Hydrolysis of Corn Straw[J];Journal of Anhui Agricultural Sciences;2007-35
3 WANG Wei-xia et al (Jiangsu Provincial Key Lab of Marine Biotechnology,Ocean College,Huaihai Institute of Technology,Lianyungang,Jiangsu 222005);Studies on Producing Chitinase with Marine Fungi HZ-01 Strain[J];Journal of Anhui Agricultural Sciences;2008-04
4 CAI Zhi-hua(Institute of Bio-pharmacies,College of Life Science,Chongqing Normal University,Chongqing 400047);Primary Exploration on the Extraction Technology of Crude Superoxide Dismutase from Chinese Quince[J];Journal of Anhui Agricultural Sciences;2008-05
5 LI Mo et al(College of Biology and Food Technology,Dalian Industrial University,Dalian,Liaoning 116034);The Optimum Conditions of Enzymes Group in the Corn Germinate Process[J];Journal of Anhui Agricultural Sciences;2008-11
6 Yuan Yi (Bioengineering Department of Anhui Agricultural University,Hefei 230036);Extraction and Purification of Coenzyme Q 10 and Cytochrome C from Pig Heart[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;1997-02
7 FENC XUNLIAN SUN QUN CAO TONGGENG LI LINGYUAN CHEN YUEZENG(Department of Biology, Peking University);THE SI STRAIN OF TETRAHYMENA FROM SHANGHAI ——TETRAHYMENA SHANGHAIENSIS sp. nov.[J];Acta Zoologica Sinica;1988-01
8 ZHANG HONG WU MEI-XI (Research Laboratory of Developmental Biology, Department of Biology, Fujian Normal University, Fuzhou 350007) Present Address: Research Laboratory of Biochemistry, Institute of Polymer Sciences, Fujian Normal University. Fuzhou 35000;REPRODUCTIVE PHYSIOLOGICAL STUDIES ON THE MALE REPRODUCTIVE CYCLE AND RENAL SEXUAL SEGMENT IN NATRIX PISCATOR:Ⅱ.EFFECT OF RENAL SEXUAL SEGMENT ON IN VITRO SPERMS, A BIOCHEMICAL ANALYSIS[J];Acta Zoologica Sinica;1994-04
9 TANG Shuang Yan ZOU GuoLin JIN JianMing (Department of Biochemistry and Biophysics, Wuhan University, Wuhan 430072, China);POLYMORPHISM OF Cu·Zn SOD IN THE BLOOD OF YAK (PO?PHAGUS GRUNNIENS)[J];Acta Zoologica Sinica;2000-04
10 CHEN Yuan-Xiao① TIAN Ming① ZHANG Wen① WANG Wen-Lin②YANG Zhao-Qing② ZHANG Nai-Guang①(①Department of Cell Biology and Genetics, Kunming Medical College,Kunming 650031; ②Department of Parasitology, Kunming Medical College,Kunming 650031,China);Nutrient Ingredient Analysis on Four Species of Land Snails in Yunnan Province[J];Chinese Journal of Zoology;2008-02
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1 Zhu Rong Gao Qi (Beijing Sport University,Beijing,China 100084);Effect of HiHiLo on Erythrocyte membrance stability and aerobic Exercise Capacity in Chinese Kickboxing Athletes[A];[C];2007
2 Wang Qing Jiang Jisen* Gan Zhifeng(Department of Physics,Center for Functional Nanomaterials and Devices East China Normal University,Shanghai 200062);Adsorption of Amino Acids On Magnetite Nanoparticles[A];[C];2005
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 Hu Zhong and He Min (Kunming Institute of Botany, Academia Sinica);STUDIES ON MABINLIN, A SWEET PROTERN FROM THE SEEDS OF CAPPARIS MASAIKAI LEVL. I. EXTRACTION, PURIFICATION AND CERTAIN CHARACTERISTICS[J];Acta Botanica Yunnanica;1983-02
2 Hu Zhong. Liang Hanxin and Lu Xiaozhu (Kunming Institute of Botany, Academia Sinica);STUDIES ON MABINLIN, THE SWEET PROTEIN FROM SEEDS OF CAPPARIS MASAIKAI—Ⅲ. STORAGE SITE AND STATE[J];Acta Botanica Yunnanica;1985-02
3 Ding Ming Hu Zhong (Kunming Institute of Botany, Academia Sinica);STUDIES ON MABINLIN, THE SWEET PROTEIN FROM SEEDS OF CAPPARIS MASAIKAI Ⅳ. STABILITY AND DENATURATION[J];Acta Botanica Yunnanica;1986-02
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 HU Xinwen GUO Jianchun ZHENG Xueqin (National Key Biotechnology Laboratory for Tropical Crops, CATAS,Danzhou,Hainan,571737,PRC);Cloning and Sequencing of cDNA Encoding Sweet tasting Plant Protein Mabinlin Ⅱ[J];LIFE SCIENCE RESEARCH;1998-03
2 Song Zongying Liu Sixin Hu Xinwen Gou Jianchuen(Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101);Splicing of and Constraction of Expression Vector of Capparis Masaikai sweet Protein Gene(MBLⅡ)[J];Biotechnology Bulletin;2006-S1
3 Liu Xinozhu Hu Zhong (Kunming Institute of Botany, Academia Sinica, Kunming);N-AND C-TERMINAL PARTIAL AMINO ACID SEQUENCE DETERMINATION OF THE SWEET PROTEINS FROM THE SEED OF CAPPARIS MASAIKAI[J];Acta Botanica Yunnanica;1988-04
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