Ofloxacin imprinted polymer applied to the solid-phase extraction of six fluoroquinolones from serum

SUN Han-wen LIU Guang-yu QIAO Feng-xia LIANG Shu-xuan (College of Chemical and Environmental Sciences,Hebei University,Key Laboratory of Analytical Science and Technology of Hebei Province,Baoding 071002,China)  

Objective:To prepare ofloxacin molecular imprinted polymer (MIP) and develop a molecularly imprin- ted solid-phase extraction (MISPE) method for determination of six fluoroquinolones in serum.Methods:The MIP was prepared using ofloxacin as template and methacrylic acid as functional monomer.Serum samples were prepared with molecularly imprinted solid-phase extraction column.All six kinds of fluoroquinolones in spiked goat serum samples were extracted with water and acetonitrile/trifluoracetic acid as washing and elution solution,respectively, followed by separation using high performance liquid chromatography.The chromatographic separation was per- formed on a Shimadzu VP-ODS column(150 mm×4.6 mm,5 μm)at temperature of 30 ℃.The mobile phase,a mixture of acetonitrile -0.02 mol·L~(-1) tetrabutl ammonium bromide (adjusted with trifluoracetic acid to pH= 2.50) (9:91),was delivered at a flow rate of 1.0 mL·min~(-1).The detection wavelength was 282 nm.Results:The MIP had a good adsorption speciality for six fluoroquinolones.For the higher affinity site,K_(d1)=1.04×10~(-4)mol· L~(-1),and Q_(max,1)=79.10 μmol·g~■.For the lower affinity site,K_(d2)=1.40×10~(-3) mol·L~(-1),and Q_(max,2)=276.79 μmol·g~(-1).Resolution of the neighbouring analytes was in the range of 1.49-2.06.Conclusion:Use of the pre- pared MIP in solid phase extraction-high performance liquid chromatography can effectively separate six fluoro- quinolones in serum samples.

【Key Words】： molecularly imprinted polymer(MIP) solid-phase extraction fluoroquinolones serum
【Fund】： 教育部博士点学科基金项目:NO.20050075003
【CateGory Index】： R96

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