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《Chinese Journal of Pharmaceutical Analysis》 2010-08
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Simultaneous quantification of seven flavonoids in eagle tea using pressurized liquid extraction and HPLC

LIU Bin(Department of Pharmacy,Anhui Provincial People's Hospital,Hefei 230001,China)  
Objective:To establish the quantitation method of eagle tea.Methods:Samples were extracted by pressurized liquid extraction(PLE).The HPLC analysis was performed on a Zorbax SB C18 column(250 mm×4.6 mm,5 μm) with gradient elution of water and methanol and flow rate of 1.0 mL·min-1;The detect wavelength and column temperature were set at 350 nm and 30 ℃,respectively.Results:Seven flavonoids,including hyperin(quercetin-3-O-β-D-galactopyranoside),isoquercitrin(quercetin-3-O-β-D-glucopyranoside),kaempferol-3-O-β-D-galactopyranoside,quercitrin(quercetin-3-O-α-L-rhamnopyranoside),astragalin(kaempferol-3-O-β-D-glucopyranoside),quercetin and kaempferol were identified in eagle tea.All calibration curves showed good linearity (r0.999) within the test ranges.The overall intra-and inter-day RSDs of seven analytes were less than 3.9% and 4.6%,respectively,and the recoveries were between 91.6% and 105.6%.Furthermore,the present method was also employed for quantification of flavonoids in eagle tea,which suggested that the contents of seven investigated flavonoids in eagle tea from different areas were obviously variant.Conclusion:This method can be used for quantification of flavonoids in eagle tea.
【CateGory Index】: R284
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