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《Chinese Journal of Pharmaceutical Analysis》 2011-01
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Preparation of baicalein molecularly imprinted monolithic columns

YANG Qing-qing1,LI Juan2,DI Bin2,3,SU Meng-xiang2,YAN Fang2,3(1.Jiangsu Institute for Food and Drug Control,Nanjing 210008,China;2.Department of Pharmaceutical Analysis,China Pharmaceutical University,Nanjing 210009,China;3.Key Laboratory of Drug Quality Control and Pharmacovigilance,Ministry of Education,Nanjing 210009,China)  
Objective:To prepare a baicalein molecularly imprinted monolithic column and study its specific recognition ability.Methods:A baicalein molecularly imprinted monolithic column was prepared by in-situ polymerization using methacrylic acid(MAA) as a functional monomer,ethylene glycol dimethacrylate(EDMA) as a crosslinking agent,the mixture of dimethylformamide(DMF) and dodecanol as porogenic reagent,and 2,2′-azobisisobutyronitrile(AIBN) as an initiator.The specific recognition ability for baicalein and wogonin was studied in different mobile phase.Effects of flow rate and temperature on retention and separation factor were investigated.Results:The best ratio of template and functional monomer was 1∶6.The baicalein molecularly imprinted monolithic polymer has specific recognition ability for the baicalein molecule.Baicalein and wogonin were separated successfully.Conclusion:The prepared molecularly imprinted monolithic column can be used as the separation media of baicalein with high affinity and good specific recognition ability.
【Key Words】: molecular imprinting monolithic column baicalein wogonin in-situ polymerization
【Fund】: 江苏省自然科学基金资助项目(No.BK2009301)
【CateGory Index】: R284
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