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《Chinese Journal of Pharmaceutical Analysis》 2011-12
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Preparation of triadimenol molecularly imprinted polymer and its application in food detection

HU Jing1,CHU Jian-gong2,GAO Wen-hui1 (1.College of Biological Science and Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China; 2.Hebei Province Meteorological Bureau,Shijiazhuang 050021,China)  
Objective:To prepare triadimenol molecularly imprinted polymer,and establish a method for determination of triadimenol residues in food.Methods:Molecularly imprinted polymer(MIP) was prepared by the way of bulk polymerization,using triadimenol as template molecule,methacrylate acid(MAA) as functional monomer,ethylene glycol dimethacrylate(EDMA) as crosslinker.MIP was used as solid phase extraction agent to treat samples.Triadimenol residues in the samples were determined by high performance liquid chromatography.Samples were analyzed on Shim VP-C18(150 mm×4.6 mm,5 μm)column,the mobile phase consisted of methanol-water(40∶ 60,v/v),the wavelength was 224 nm.Results:The results showed that the linear range of triadimenol was 0.1-200 μg·mL-1,linear correlation coefficient was 0.9998;the detection limit was 0.5 μg·g-1;under 2 μg·g-1 and 10 μg·g-1 added levels,the average recoveries were between 79.3%-86.0%,and the relative standard deviations(RSDs) were ≤2.3%(n=5).Conclusion:This method has good sensitivity and reproducibility,is an effective method of detecting triadimenol fungicide residues in food.
【Key Words】: fungicide triadimenol vestigital detection molecular imprinting adsorption property molecularly imprinted solid phase extraction high performance liquid chromatography
【Fund】: 河北省科技厅重大科技攻关项目(09227134D);; 河北省科技支撑计划项目(10276902D);; 河北省自然科学基金资助项目(B2008000669)
【CateGory Index】: TS207.3
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