Detection of Pneumonia Chlamydiae in the Peripheral Blood Mononuclear Cell of Coronary Heart Disease by Indirect Immunofluorescence and Nest-PCR
CAI Junming*,LI Tao,ZHANG Guoliang, et al.Shenzhen City Pingshan People’s Hospital,Guangdong Province 518000
Objective:To detect pneumoniae chlamydia in peripheral blood mononuclear cell (PBMC).Methods:The PBMC were isolate form 3 ml of the whole blood specimen by low permeate hemolysis method. The inclusion bodies was determined by direct immunofluorescence(DIF) and the DNA by nest-PCR in 150 cases with coronary heart disease( CHD) and 55 non CHD(control group,CG).Results: Pneumoniae chlamydia Inclusion bodies was detected in 20(13.3%) of 150 patients with coronary heart disease and in 1(1.8%) of 55 in control group.Cpn DNA positive rats was 32.7%(49/150) in CHD and 1.8%(1/55) in control group .The positivity rates of chlamydia pneumoniae inclusion bodies and the Cpn DNA in CHD groups were significantly higher than those in control group. Conclusion:Pneumoniae chlamydia inclusion bodies and DNA were present in PBMC among the significant proportion of CHD patients. PBMC sample can be collected easily and trustworthily.The positive result in PBMC is showing evidence for the presence of Cpn and is infecting.