Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Pharmaceutica Sinica》 2003-01
Add to Favorite Get Latest Update

Preparation and characterization of monoclonal antibody against marine sulfated polysaccharide drug poly-mannaguluronic acid

CHEN Kun 1, XIN Xian-liang 1, GENG Mei-yu 1, ZHU Jian-chun 2, YANG Ming 2, LI Yong 2 (1. Marine Drug and Food Institute, Ocean University of Qingdao, Qingdao 266003, China; 2. Biologics Institute of Changchun, Changchun 130012, China)  
Aim To prepare and characterize the monoclonal antibody against poly-mannaguluronic acid (911). Methods A hybridoma cell line was obtained by cloning for 3 times those cells that secret antibody against 911 after the fusion of NS-1 myelome cells with spleen cells from Balb/c mice immunized with a 911-bovine serum albumin conjugate, prepared by reductive amination. Hybridoma was inoculated to Balb/c mouse to induce ascites. The antibody was purified by ammonium sulfate and Protein A - Sepharose CL-4B. DD3 was confirmed to be fusion cells after determination of DNA content with flow cytometry. Competitive inhibitory test and Biacore confirmed the cross-reactivity of the antibody with other endogenous polysaccharides or with alginic acid sodium. Igs′ classes and subclasses were identified by Isostrip TM method. The affinity of DD3 was verified by ELISA. Results A hybridoma cell line secreting monoclonal antibody against 911 (marine sulfate polysaccharide) named DD3 was obtained. The DD3 ascites contained specific antibody with titer over 1.0×10 5. There was no cross-reactivity of these antibodies with other endogenous polysaccharides or with alginic acid sodium. The immunoglobulin subclass of DD3 was IgG2a, κ type. The affinity of DD3 was 2.0×10 8 L·mol -1 . Conclusion One hybirdoma line (DD3) secreting monoclonal antibody against 911 was established to provide a potential method for the pharmacokinetic study of 911.
【Fund】: 国家自然科学基金重点项目 (3 0 13 0 2 0 0 ) ;; 国家自然科学基金资助项目 (3 0 0 70 0 694)
【CateGory Index】: R392
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 3 Hits
1 ;Research development of monoclonal antibody of saccharide[J];Marine Sciences;2006-10
2 WANG Xiao-feng ZHENG Qing(Biopharmaceutical R&D Center of Jinan University, Guangzhou 510630,China);The Methodology and Experimental Degsin in Pharmacokinetics Study of Biotechnological Drugs[J];Progress In Biotechnology;2004-02
3 XIE Tao-yin, ZHAO Hu, GAO Xiang-dong * (School of Life Science & Technology, China Pharmaceutical University, Nanjing 210009, China);Advances in the Research on the Monoclonal Antibody against Polysaccharides[J];Progress In Pharmaceutical Sciences;2005-09
Chinese Journal Full-text Database 1 Hits
1 HAN Wei, ZHANG Chun-qing, QI Ji-ping, LI Dan-yang Department of Pathology, The First Hospital of Harbin Medical College, Harbin 150001, ChinaCorresponding Anthor: QI Ji-ping;Expression of IGF-1 in Reactive and Neoplastic Astrocytes: An Immunohistochemical Study Using Tissue Microarray Techniques[J];Cancer Research on Prevention and Treatment;2006-06
Chinese Journal Full-text Database 10 Hits
1 Zhang Xuan,Zhang Ming,Su Xu,Liu Jianxiang,Li Fenglan,Ma Xiaohui (MH Radiobiology Research Unit);Experimental study on pharmacokinetics of genetic recombinativehuman basic fibroblast growth factor (rhbFGF)[J];JOURNAL OF NORMAN BETHUNE UNIVERSITY OF MEDICAL SCIENCE;1999-03
2 ZHAO Ning 1, ZHANG Ying Qi 1, WANG Zeng Lu 1, GAO Lian Yong 2, GU Yi Bao 2, LI Quan Sheng 2, CAI Yong Ming 2, ZENG Yong 2, CHEN Zheng Min 2, LIU Chang Xiao 2 1Biotechnology Center, Administration of Science Research, Fourth Milit;Pharmacokinetics of new recombinant human tumor necrosis factor alpha in mice[J];Journal of The Fourth Military Medical University;2002-12
3 CHEN Jiong-ran, HU Ting-jun (Lanzhou Institute of Animal & Veterinary Pharmaceutics Science,Chinese Academy of Agricaltural Sciences,Lanzhou,Gansu,730050,China);Progress on Antirival Activities of Sulfated Polysaccharides[J];Progress In Veterinary Medicine;2005-04
4 Ma Dingyuan, Chen Jun, Li Ping * (Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038) Hu Zhuoyi (Department of Biochemistry, China Pharmaceutical University, Nanjing 210009);Analysis of Monosacchaaride Compositions in Polysaccharides by Pre-column Derivatization High Performance Liquid Chromatography[J];Chinese Journal of Analytieal Chemistry;2002-06
5 Zhang Zhenqing,Yu Guangli,Zhao Xia,Li Qian,Guan Huashi(Institute of Marine Drug and Food,Ocean University of China,Qingdao,266003);Thin Layer Chromatography Analysis of Several Uronates and Their Oligosaccharides[J];Chinese Journal of Analytical Chemistry;2005-12
6 LI Bo~(1*), GU Xiao-hong~(2), XU Shi-ying~(1) (1. School of Food Science and Technology, Southern Yangtze University, Wuxi,Jiangsu 214036; 2. Analysis Center of Southern Yangtze University, Wuxi,Jiangsu 214036);Determination of the Position of Sulphate Group in Sulphated Polysaccharides[J];Journal of Analytical Science;2004-05
7 ZHANG Li-Na; DING Qiong; ZHANG Ping-Yi(Deparment of Chemistry, Wuhan University, Wuhan, 430072)FENG Han-Qiao(Wuhan Institute of Physics, Academia Sinica, Wuhan, 430072);Isolation and Structural Analysis of Polysaccharides from the Sclerotium of Poria Cocos Wolf[J];CHEMICAL RESEARCH IN CHINESE UNIVERSITIES;1997-06
8 DING Qiong,ZHANG Lina (Department of Chemistry,Wuhan University,Wuhan 430072) CHEUNG Peter Chi Keung (Department of Biology,The Chinese University of Hong Kong,Hong Kong);ISOLATION AND STRUCTURAL ANALYSIS OF POLYSACCHARIDES FROM PORIA COCOS MYCELIA[J];ACTA POLYMERICA SINICA;2000-02
10 LI Dong-xia,ZHANG Shuang-quan,LIU Ping Life Science College of Nanjing Normal University,Nanjing 210097,China;Study on the Purification of SCAMP and the Preparation of Its Sulfated Polysaccharides[J];Spectroscopy and Spectral Analysis;2002-01
【Secondary References】
Chinese Journal Full-text Database 5 Hits
1 WANG Jing-jing,YANG Chun-wen,ZHANG Ying-ying,DONG Ying-wei,DU Dai-di(Mudanjiang Normal University,Mudanjiang,Heilongjiang 157012);Optimize and Search on Process for Extraction of Polyporus umbellatus Polysaccharides[J];Northern Horticulture;2010-20
2 PENG Zhen-fei,PENG Xu,MENG Chun(College of Biological Science and Technology,Fuzhou University,Fuzhou,Fujian 350002,China);Study on mechanism of zwitterionic polysaccharide in humoral immune response[J];Journal of Fuzhou University(Natural Science Edition);2007-02
3 YU Yuan 1,2 LIU Zhong-rong 2 YU Xiao-qi 1 WU Qia-qing 2(1 College of Chemistry Sichuan University Chengdu 610064,China)(2 Chengdu DI-AO pharmaceutical Group Co. Ltd Chengdu 610041,China);Perspectives of the Genetic Recombinant Medicine on Its Research and Development[J];Progress In Biotechnology;2005-10
4 WU Lei,CHEN Xi-jing(Center of Drug Metabolism and Pharmacokinetics;China Pharmaceutical University,Nanjing 210009,China);Recent Development in Pharmacokinetics of Biotech Drugs[J];Progress in Pharmaceutical Sciences;2008-05
5 LI Pei-shan1,ZHANG Chun-yan2,LI Shi-jun1,CHEN yan2,XIANG Mei-juan1,LI Fang-he1,ZHANG Hong1(1.Department of Pharmacy,the First Clinical Medical Collegy,Wuhan University,Wuhan 430060,China;2.Central of Medicine,Tongji Hospital Affiliated with Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China);Preparation of Monoclonal Antibody Against Neisseria Meningococcal Serogroup A Polysaccharide(GAMP) by Using the Conjugate Vaccine of both GAMP and Tetanus Toxoid[J];Herald of Medicine;2011-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved