Simultaneous determination of 4 prenylflavonoids of Chuankezhi injection in rat plasma by LC-MS/MS
XU Shun-jun;ZHU Ya-ling;YU Jie-jing;FENG Yi;SUN Shuai;XU Yi-juan;YANG Liu;Guangzhou Im Vin Pharmaceutical Co.Ltd.;Guangdong Second Traditional Chinese Medical Hospital;Guangdong Provincial Hospital of TCM, Guangdong Provincial Academy of Chinese Medical Sciences;
A quantitative method for epimedin A, B, C and icariin in rat plasma was established using LC-MS/MS after intermuscular administration of Chuankezhi injection to rat. Chromatographic separation was performed on an Agilent Eclipse XDB-C_(18) column(150 mm × 2.1 mm, 5.0 μm) at 40 ℃. Mobile phase consisted of acetonitrile-0.1% formic acid in water(35∶65), and the flow rate was 0.22 m L·min~(-1). The LC effluent was detected and analyzed using an ESI-triple quadrupole tandem mass spectrometer under the multiple reaction monitoring(MRM) in the negative ion mode. The plasma samples were treated with solid phase extraction prior to LC-MS/MS analysis. As a result, all of the four analytes displayed a good linearity over the concentration of 1-1 000 ng·m L~(-1). The RSDs of intra-day and inter-day assays were less than 5.99% and 10.16%, respectively. The relative recovery of each analyte was between 88.1%-101.1% with RSD 7.9% and the absolute recovery was between 72.0%-86.6%(RSD 6.3%). In conclusion, the established method shows good specificity, sensitivity and efficiency for quantifying the four flavonoid glycosides contained in rat plasma.
【CateGory Index】： R285.5