Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
Add to Favorite Get Latest Update


He Jianhua(He JH) and Xu Chengxiong(Xu CX) (Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050)  
AIM: To study the mechanism of inhibitory action of howiinol A(GHM 10) on synthesis of DNA in cancer cells. METHODS: Single cell gel electrophoresis was used to detect the DNA molecular damage induced by GHM 10 in L1210 cells. Alkaline elution technique was used to investigate the effect of GHM 10 on the length of DNA strands of L1210 cells. Dehelix effect of GHM 10 on superhelix pUC 18 DNA was used to estimate its influence on the activity of DNA topoisomerase II. RESULTS: After L1210 cells were treated with 4 and 10 μg·ml -1 of GHM 10 for 4 5 h, comet like tails could be seen in cells under a fluorescence microscope after electrophoresis, indicating that DNA damage was induced by GHM 10. DNA single strand breaks were demonstrated when L1210 cells were treated with GHM 10 in concentrations of 4 and 25 μg·ml -1 for 5 h. When L1210 cells or protein isolated from L1210 cells were treated with GHM 10, the activity of DNA topoisomerase II was inhibited. CONCLUSION: GHM 10 induces damage to DNA molecules in L1210 cells and inhibits the activity of topoisomerase II both inside and outside the cells.
【CateGory Index】: R963
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 2 Hits
1 Liu Jie * Ji Liangnian(The Key Laboratory of Gene Engineering of Education Ministry, School of Chemistry and Chemical Engineering, Zhongshan University, Guangzhou 510275, China)Mei Wenjie (Department of Pharmaceutical, Guangdong Pharmaceutical University, Guangzhou 510315, China);The Development of Ruthenium Complexes as Anticancer Drugs Activation and Anticancer Mechanism[J];化学进展;2004-06
2 ;Advances in Study on Anti - tumor Mechanism of Traditional Chinese Medicine[J];中医药学刊;2003-03
Chinese Journal Full-text Database 10 Hits
1 MEI Wen-Jie 1, LIU Jie 1, LI An-Xing 2, HUANG Qi-Wen 1, JI Liang-Nian 1* (1. The State Key Laboratory of Optoelectronic Materials and Technologies, College of Chemistry and Chemical Engineering; 2. College of Life Science, Zhongshan University, Guangzhou 5102752, China);Investigation on the Interaction of Ruthenated Porphyrin [Ru(bpy)_2(MPyTPP)Cl]~+ with Calf Thymus DNA[J];高等学校化学学报;2002-11
2 Wang Zhongming Ji Liangnian ** (The Key Laboratory of Gene Engineering of Education Ministry; School of Chemistry and Chemical Engineering, Zhongshan University, Guangzhou 510275, China);Progress of Ruthenium-Based Antitumor Complexes[J];化学进展;2002-04
3 LIU Jie 1 , XU Dong Hui 2, MEI Wen Jie 1, PU Han Lin 2, HUANG Jin Wang 1, JI Liang Nian 1* (1. State Key Laboratory of Ultrafast Laser Spectroscopy, Department of Chemistry; 2. Department of Pharmacy, Zhongshan University, Guangzhou 510;Synthesis, Anticancer Activity and Mechanism of Water-soluble Porphyrin and Metalloporphyins[J];高等学校化学学报;2001-09
4 LIU Jie 1, ZHANG Qian Ling 1, WANG Li Jun 2 WANG En Bo 3, HUANG Jin Wang 1, JI Liang Nian 1 [WT5”BX](1. State Key Laboratory of Ultrafast Laser Spectroscopy, Department of Chemistry, Zhongshan University, Guangzhou 510275, China;Synthesis, Properties and Antiviral Activity of Keggin Molybdogemnium Heteropoly Blues[J];高等学校化学学报;2000-12
5 LI Wei,LI Yan ②,JIN Xiong Jie ③.Department of Hematology, First Teaching Hospital of Norman Bethune University of Medical Science, Changchun 130021;Antitumor activity and immunoregulatory effect of triterpenes isolated from betula platyphylla[J];中国免疫学杂志;2000-09
6 Mao Haiting,Zhang Ling, Wang Yun,Li Xiaobing (Institute of Basic Medical Sciences,Shandong Academy of Medical Sciences, Jinan 250062);Experimental Studies of Icariin on Anticancer Mechanism[J];中药材;2000-09
7 WANG Jin,WANG Xiang hong, Lillian SY CHOW,et al(The University of Hong Kong, Pokfulam Road,Hongkong, China);Effects of gossypol in inducing apoptosis of human colon cancer cell lines[J];中国癌症杂志;2000-04
8 ZHANG Yan, JIANG Ji kai*, LIU Xiao shan, et al Department of Clinical Biochemistry, Chongqing University of Medical Sciences, Chongqing 400016, P R China;Differentiation and apoptosis in K562 erythroleukemia cells induced by matrine[J];癌症;2000-08
9 MENG Ling hua, JIANG Chao, DING Jian*, et al Shanghai Institute of Meteria Medical, Chinese Academy of Sciences, Shanghai 200031, P R China;Inhibition of DNA topoisomerases and direct DNA breakage by extract of spirulina plantensis geitl[J];癌症;2000-08
10 Liang Yong; Yang Yiming; Yuan Shulan; Liu Ting; Jia Yongqian; Xu Caigang; Niu Ting; Qin Hui; Qin Ping(Department of Hematology, The First Affiliated Hospital, WCUMS, Chengdu 610041);Terminal Differentiation of Human Acute Promyelocytic Leukemia (APL) Cells Induced by Tanshinone Ⅱ A in Primary Culture[J];华西医科大学学报;2000-02
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 Cao Yaping;Yi Cenlan;Liu Hongmei;Li Haixia;Zuo Jianli;Yuan Zeli;School of Pharmacy,Zunyi Medical University;;Synthesis,and interaction with DNA of a new oxovanadium(Ⅳ)complex containing L-Methionine Schiff base and 1,10-phenanthroline[J];遵义医学院学报;2015-06
2 CAO Ya-ping;YI Cen-lan;LIU Hong-mei;LI Hai-xia;ZUO Jian-li;YUAN Ze-li;School of Pharmacy,Zunyi Medical University;;Synthesis and Anticancer Activities of Novel Oxovanadium( Ⅳ)Complexes[VO( dtbsal-met)( phen) and VO( naph-met)( phen) ][J];合成化学;2015-12
3 Zhang Min;Xu Yuanhong;Dong Bingbin;Dept of Clinical Laboratory,The First Affiliated Hospital of Anhui Medical University;Dept of General Surgery,The First Affiliated Hospital of Anhui Medical University;;Ru complex as tumor inhibitor by regulating FSCN1 in gastric cancer cells[J];安徽医科大学学报;2015-12
4 HU Jiu-rong;TONG Huai-duo;LI Yan-hong;WANG Yong-hua;PENG Guan-yin;XU Wei;Shangrao Normal University;Middle School of Shangrao;;Progress on Research of Half-sandwich Ruthenium Compounds Based on Chalcogenolate Ligands[J];上饶师范学院学报;2013-06
5 LIU Ai-rong,LI Xian-chuan,MIAO Qian(Wenzhou University,Zhejiang Wenzhou 325035,China);Electrochemical Properties of Tetramethylpyrazine Bridged Arene-Ruthenium Complex[J];辽宁化工;2012-08
6 Li Ning1,Chen Lanmei1,Zhang Haitao2,Jia Zhenbin1,Luo Hui1(1School of Pharmacy,2Institute of Biochemistry and Molecular Biology,Guangdong Medical College,Zhanjiang,Guangdong Province,524023,China);Synthesis of ruthenium complex [Ru(MeIm)_4(bpy)]~(2+) and its effect on proliferation in human liver cancer cell lines[J];第三军医大学学报;2012-11
7 Liang,Yaohuaa,b,c Liu,Yanzhongc Bi,Weic Liang,Guogang*,a,b,c(a State Key Laboratory of Quality Research in Chinese Medicine,Faculty of Chinese Medicine,Macau University of Science and Technology,Avenida Wai Long,Taipa,Macau)(b Macau Institute for Applied Research in Medicine and Health,Macau University of Science and Technology,Macau)(c Institute of Chinese Material Medica,China Academy of Chinese Medical Science,Beijing 100700);Influence of Ligand Structure on the Hydrolysis and Stability of NAMI Derivatives Containing 2-Methyl imidazole and N-Ethyl imidazole[J];化学学报;2012-07
8 ZHAO Li-hui1,ZHANG Yi-zhe2,DU Juan1,HAN De-ming1,TIAN Jian1,SUN Xiu-ping1(1.International Joint Research Center for Nanophotonics and Biophotonics, Changchun University of Science and Technology,Changchun 130022,China; 2.Bioengineering Department,Zhengzhou University,Zhengzhou 540001,China);Studies of activities component of antidepressie and anticancer on Valeriana of ficinalis L.[J];东北师大学报(自然科学版);2011-03
9 TAN Nian-yuan,TAN Ling-ling,CHEN Li-xin,YAN Wei-wei,HUANG Sai-jin(College of Chemistry and Chemical Engineering,Hunan Institute of Engineering,Xiangtan 411104,China);Synthesis,Characterization and DNA Binding Studies of a New Polypyridine Cobalt(Ⅲ) Mixed Ligand Complex[J];湖南工程学院学报(自然科学版);2011-02
10 Zhang Xiaonian Liu Yanan Yang Xiaoxin Liu Jie**(Department of Chemistry,College of Life Science and Technology,Jinan University,Guangzhou 510632,China);Apoptotic Signal Pathways of Tumor Cells Induced by Ruthenium Complexes and Its Mechanisms[J];化学进展;2011-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved