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Sun Lan(Sun L), Wang Qing(Wang Q) and Liu Jingsheng(Liu JS) (Institute of Basic Medical College, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100005)  
AIM: To study the effects of estrogen on stimulating bone formation in osteoblast like cell line TE85. METHODS: Utilizing methods of 3H thymidine incorporation for cell proliferation, 3H proline incorporation for collagen synthesis, ultraviolet spectrophotometry for measurement of alkaline phosphatase (ALP) activity, radioimmunoassay for bone gla protein (BGP) content assay, and radio ligand binding for estrogen receptor assay. RESULTS: Cells were incubated with various concentrations (from 0 01 to 10 nmol·L -1 ) of 17β estradiol (E 2) for 48 h and 72 h, 3H thymidine incorporation into DNA was increased gradually while 3H proline incorporation was enhanced in the same fashion. E 2 at the same dose increased the intracellular ALP activity and BGP content at the same time. In ligand receptor binding experiment, the K D of 3H E 2 saturation test was 2 59×10 -10 mol·L -1 in TE85 cells and the receptor number was found to be 262 7±64 6 site per cell. The IC 50 of 4 OH tamoxifen and ICI 182,780 were 0 99×10 -8 mol·L -1 and 8×10 -10 mol·L -1 , respectively. CONCLUSION: E 2 improved bone formation by ways of stimulating cell proliferation, enhancing collagen synthesis, ALP activity, and BGP content. The effects of E 2 were performed by the mediation of estrogen receptor.
【Fund】: 国家自然科学基金
【CateGory Index】: R962
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