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Guo Ying(Guo Y), Hu Yufang(Hu YF) and Cheng Guifang(Cheng GF) (Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050)  
AIM: To establish the model of determining nuclear factor κB(NF κB) which is stimulated by different inflammatory irritants in inducible peritoneal macrophages from mouse and investigate the inhibitory effect of aspirin on NF κB activation using this model. METHODS: Lipopolysaccharide(LPS) and phorbol 12 myristate, 13 acetate(PMA) were used as irritants. Electrophoretic mobility shift assay (EMSA) was used as the determination method. RESULTS: LPS(μg·mL -1 , 3 μg·mL -1 ) and PMA (1 ng·mL -1 ) was shown to increase the content of NF κB in inducible peritoneal macrophages from the mouse. After the macrophages were treated with aspirin(10 -7 mol·L -1 , 10 -6 mol·L -1 , 10 -5 mol·L -1 ), the increase was inhibited. CONCLUSION: The model of determining NF κB stimulated by LPS and PMA can be used in researching mechanism of non steroidal anti inflammatiory drugs.
【Fund】: 国家自然科学基金
【CateGory Index】: R965
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