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《ACTA PHARMACEUTICA SINICA》 1999-08
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INDUCTION OF NF κB IN MOUSE PERITONEAL MACROPHAGES BY INFLAMMATORY IRRITANTS

Guo Ying(Guo Y), Hu Yufang(Hu YF) and Cheng Guifang(Cheng GF) (Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050)  
AIM: To establish the model of determining nuclear factor κB(NF κB) which is stimulated by different inflammatory irritants in inducible peritoneal macrophages from mouse and investigate the inhibitory effect of aspirin on NF κB activation using this model. METHODS: Lipopolysaccharide(LPS) and phorbol 12 myristate, 13 acetate(PMA) were used as irritants. Electrophoretic mobility shift assay (EMSA) was used as the determination method. RESULTS: LPS(μg·mL -1 , 3 μg·mL -1 ) and PMA (1 ng·mL -1 ) was shown to increase the content of NF κB in inducible peritoneal macrophages from the mouse. After the macrophages were treated with aspirin(10 -7 mol·L -1 , 10 -6 mol·L -1 , 10 -5 mol·L -1 ), the increase was inhibited. CONCLUSION: The model of determining NF κB stimulated by LPS and PMA can be used in researching mechanism of non steroidal anti inflammatiory drugs.
【Fund】: 国家自然科学基金
【CateGory Index】: R965
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【Co-references】
Chinese Journal Full-text Database 10 Hits
1 SU Dong, TAO Guo cai, LI Kun (Department of Anesthesiology, Southwest Hospital, the Third Military Medical University,Chongqing 400038);Experimental study of aprotinin pretreatment protecting the liver from ischemia-reperfusion injury in rats[J];Chongqing Medical Journal;2002-08
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5 Wang Qingduan,Jang Jinhua,Sun Wenxin,Xia WeiHenan Institute of Medical Sciences,Zhengzhou 450052;The Correlationship between reversing effect of ligustrazine(LIG) on multidrug resistance and content of GSHPx in EAC/ADR cell lines[J];JOURNA OF HENAN MEDICAL UNIVERSITY;1997-04
6 WANG Yong yi, XUE Song, LIU Sha, XIAO Ming di (Department of Cardiovascular Surgery, Shanghai First People's Hospital,Shanghai 200080,China);Aspirin reduces myocardial ischemia/reperfusion injury by preventing activation of transcription factor NF-κB[J];Bulletin of Medical Postgraduate;2003-02
7 ZHENG Xiaoling,YI Jiling,CHEN Limo.Center of Hepatic Surgery,Tongji Hospital Affiliated to Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China;Delayed protective effect of liver ischemic preconditioning on liver ischemia-reperfusion injury and initial inquiry into the protective mechanism[J];Journal of Clinical Surgery;2002-S1
8 HAN Qun-ying, GU Zhen, WANG He-ming, PENG Tao, SU Dian-san (Department of Anatomy and Histology and Embryology, NJMU, Nanjing 210029, China);The Relationship between Nitric Oxide Synthase Activity and Cerebral Apoptosis after Focal Cerebral Ischemia in Rats[J];Acta Academiae Medicinae Nanjing;2002-02
9 WU Ya-guang,WU Tai-huang,MU Qing-ling,LU Jun,XU Jian,SU Zhong-xue Department of Hepatobiliary Surgery,Shandong Provincial Hospital(Jinan 250021,China);Involvement of nitric oxide(NO) and endothelin(ET) in ischemia-reperfusion injury and its role in preconditioning[J];Chinese Journal of Current Advances in General Surgery;2003-04
10 LIU Ji ming 1, ZHU Xing guo 2, ZHU Hua ting 1, ZHANG Yi 1, LI De chun 2, KANG Su ya 2, ZHANG Xue guang 1 (1 Department of Immunology, Suzhou Medical College, Suzhou 215007; 2 Department of Surgery, The First Affiliated Hospital of Suzhou Me;Inhibition of Neutrophil-mediated Ischemia/Reperfusion Injury in Rabbit Liver by Anti-IL-8 Antibody[J];Shanghai J.of Immunology;2001-01
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