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《Journal of Molecular Diagnostics and Therapy》 2016-03
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Evaluation and application of a domestic NAT kit used for blood screening

CHEN Zhizhong;LI Jiemin;LIAO Yangxun;LIANG Jianfeng;YU Wenchao;TANG Xiaoying;LIANG Liting;HUANG Juming;LU Qianwen;CHEN Shangliang;Zhaoqing Blood Center;  
Objective To evaluate the capability of a domestic blood screening NAT kit.Methods The sensitivity,specificity,repeatability,anti-interference ability,and anti-pollution ability of the kit were analyzed. Results The sensitivity of HBV DNA,HCV RNA and HIV RNA detection were not more than 50 IU/m L,50 IU/m L and 100 IU/m L respectively,and specificity,repeatability,anti- pollution ability met the demands for blood nucleic acid test. There was no interference due to fat particles in the analyses of HBV DNA,HCV RNA and HIV RNA in mini pool of 8 samples model,but HIV RNA was not detected in ID- NAT model due to fat particles. The detection of those 3 marks was interfered with by hemolysis.Conclusions The sensitivity,specificity,repeatability,anti-interference ability and anti-pollution ability of the kit could meet the demands for blood screen,but the interference from fat particles or hemolysis is a concern for the detection capability for HBV DNA,HCV RNA and HIV RNA.
【Fund】: 广东省医学科学技术研究基金项目(A2015238);; 肇庆市科技创新计划(2014E1814)
【CateGory Index】: R446.6
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