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《Journal of Molecular Diagnostics and Therapy》 2018-05
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Detection of methylation of SOX1 and VIM promoters in tumor tissues and serum from patients with endometrial carcinoma and its clinical significance

SHU Xinhong;FAN Hongli;LI Xiaoyan;Department of Gynecology,the People's Hospital of Yingshan County;  
Objective To detect the methylation status of sex-determining region Y-box protein 1(SOX1)and vimentin(VIM)promoters in serum from patients with endometrial carcinoma(EC),and to explore its clinical significance. Methods A total of 120 patients with EC who were admitted to Yingshan County People's Hospital from July 2013 to December 2015 were enrolled in the observation group,while 50 patients with functional uterine bleeding were recruited as the control group.Methylation-specific PCR(MSP)was used to detect the methylation status of the promoters of SOX1 and VIM gene in serum and tumor tissues.The relationship between the promoters of SOX1 and VIM methylation and clinicopathological features of EC patients was analyzed.The sensitivity,specificity and accuracy of SOX1 and VIM alone or combined detection were also analyzed. Results(1)The methylation rates of the promoter of SOX1 gene in serum and tumor tissues from EC patients(71.61% and 95.83%) were significantly higher than that in the control group(10.00% and 2.00%)(P0.05).(2) The methylation rates of the promoter of VIM gene in serum and tumor tissues from EC patients(60.83% and 94.17%) were significantly higher than that in the control group(12.00% and 4.00%),(P0.05).(3)The promotor methylation levels of SOX1 and VIM gene were correlated with the pathological staging,myometrial invasion depth and lymph node metastasis in patients with EC patients(P0.05),and not associated with the patient's age or tumor type of HCC(P0.05).(4) The sensitivity of serum of the promoter of SOX1 and VIM methylation alone and combined diagnosis was 71.67%,60.83%,81.67%,and the specificity was 90.00%,88.00%,84.00%,and the accuracy was 77.06%,68.82%,86.47%,respectively.Compared with SOX1 and VIM alone,the sensitivity and accuracy of combined diagnosis significantly increased(P0.05).There was no significant difference in the specificity of different diagnostic methods(P0.05). Conclusion The methylation of the SOX1 and VIM genes are potential markers for EC. The combined detection of the SOX1 and VIM gene methylation in promoters can be used for clinical EC screening and early diagnosis.
【CateGory Index】: R737.33
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