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《Journal of Molecular Diagnostics and Therapy》 2018-05
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Study on the drug resistance mechanism of carbapenem-resistant Providencia rettgeri

JIA Qinmei;SUN Le;MAN Baohua;Department of Clinical Laboratory,the Third People's Hospital of Yunnnan Province;Biochemistry Laboratory,Kunming Medical University;  
Objective To analyze the drug resistance and the enzyme production of a strain of Providencia rettgeri producing KPC-2 isolated from foot infection. Methods The strain was cultured,isolated and identified.The in vitro drug sensitivity test was performed by MIC and KB methods.The carbapenemase phenotype analysis was carried out by modified Hodge experiment and EDTA synergistic experiment.The carbapenemase genotype was further determined by PCR.Results The isolated Providencia rettgeri was resistant to quinolones and β-lactams(including carbapenems),and sensitive to amikacin.The modified Hodge test was positive and the EDTA-disk synergy test was negative.Only the KPC-2 gene was detected and the IMP,VIM,NDM-1 and OXA-48 carbapenemase genes were no detected.Conclusion The clinical isolate of this strain of KPC-2 carbapenemase,which is a rare strain of carbapenemase,requires close monitoring the carbapenemase-producing strain and adopts infection control measures to prevent the drug-resistance genes transmitted between bacteria.
【Fund】: 云南省科技厅应用基础研究联合专项资金项目(201501UC00015)
【CateGory Index】: R446.5
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