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《China Medical Equipment》 2015-01
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Effect of PIAS3 over expression on proliferation in adenocarcinoma A549 cells and the significance

YU Qing-hua;LI Feng-sheng;HUANG Nai-xiang;Department of Thoracic Surgery,307 Hospital of PLA;  
Objective: To explore the effect of PIAS3 on proliferation of lung adenocarcinoma A549 cells, in order to provide a foundation for the further study in which a adeno-associated virus(AAV) containing PIAS3 gene driven by a radiation response promoter plus radiotherapy will be used to treatment lung adenocarcinoma. Methods: The growth and proliferation of A549 cells transfected with p CMV-Sport6-PIAS3 plasmid were detected by MTT assay and cloning forming efficiency assay. The nucleus translocation of p-STAT3 was detected by immunofluorescence staining after CMV-Sport6-PIAS3 was co-transfected with PRC-CMV, PRC-STAT3 or PRCSTAT3-CT. Results: The growth and cloning form of A549 cells were significantly inhibited by PIAS3 overexpression. The result of immunofluorescence staining indicated that the nucleus translocation of p-STAT3 as significantly blocked by transfection with p CMV-Sport6-PIAS3, even after co-transfection with STAT3 or STAT3-CT. Conclusion: The overexpression of PIAS3 could inhibit the nucleus translocation of STAT3, which subsequently blocked the function of STAT3 and decreased the ability of growth and proliferation of A549 cells.
【Fund】: 国家自然科学基金(31340051)“腺病毒Ad-pig3RRP-PIAS3对辐射诱导肺腺癌细胞转移的抑制作用及其机制研究”;国家自然科学基金(81202151)“低剂量辐射对树突状细胞迁移能力的影响及其分子机制研究”;国家自然科学基金(81001216)“NOK激酶促进肿瘤细胞周期进程及提高肿瘤细胞辐射敏感性分子机制研究”;; 全军医学科技“十二五”科研项目(CWS12J082)“快速PCC-FISH方法作为辐射生物剂量计的研究”
【CateGory Index】: R734.2
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