Detection of D-lactic acid and endotoxin and effect of dietary fiber complex on intestinal mucosal barrier in rats with portal hypertension
QIAO Xiaodong;WU Hua;Shanxi Medical University;Department of General Surgery, the First Clinical Medical College of Shanxi Medical University;
Objective To research the protective effect of dietary fiber complex(DFC) on intestinal mucosal barrier in rats with portal hypertension. Methods The healthy female Wister rats with portal hypertension which were established by CCl4olive oil complex method were randomly divided into four groups: group A(simple model group), group B(the rats were fed with Nutrison), group C(the rats were fed with Nutrison and DFC, SDF∶IDF=1∶2), group D(the rats were fed with Nutrison and DFC, SDF∶IDF=2∶1). At 1, 3, 7 d after modeling, 3, 3, 4 rats in each group were randomly selected ileum tissue to observe the pathological changes in the light microscope. Blood samples were taken for D-lactic acid content and endotoxin content. And the normal control group was sampled at 7 d as the tested group. Results The organizational structure of intestinal mucosa of rats in each experimental group were damaged, the control group were no damage. The levels of D-lactic acid and endotoxin in the blood were significantly higher than those in the control. At 1 d after modeling, the differences were not statistically significant in tested groups(plasma D-lactate: F = 0.579, P = 0.633; endotoxin: F = 0.108, P = 0.955). At 3 d and 7 d, the differences in tested groups were statistically significant(plasma D-lactate 3 d: F = 39.622, P 0.001, 7 d: F = 203.834, P 0.001; endotoxin 3 d: F = 95.863, P 0.001, 7 d: F = 2742.321, P 0.001). Compared with group A, the differences in the levels of D-lactic acid and endotoxin in the group B, C and D in 3, 7 d were significant(P 0.05), the levels significantly reduced. Compared with B, the differences were significant in the group C and D in 3, 7 d(P 0.05). Compared with group C, the difference were significant in the group D in 7 d(P 0.05). Conclusion DFC has a protective effect on gut barrier in rats with portal hypertension.