Interference of Chlorogenic Acid on TLR2 Signal Pathway in HSV-1 Infected Microglia
DENG Na;WANG Jintang;Department of Pediatrics,the People's Hospital of Shiyan City,Hubei Province;
Objective To investigate interference of chlorogenic acid( CGA) on TLR2 signal pathway in herpes simplex virus( HSV)-1-induced responses in BV2 microglia. Methods The cellular model was established with BV2 cells stimulated by HSV-1 and then treated with CGA at different concentrations.The mRNA expression of Toll-like receptor( TLR)-2 and Myd88 was assayed by real-time quantitative PCR( RT-PCR),and the protein expression was assayed by Western blotting.IL-6 levels in the serum were tested by ELISA and RT-PCR. Results TLR2,Myd88 and IL-6 were significantly higher in the model control group than in the normal control group( P0.05).After CGA treatment,TLR2,Myd88,IL-6 were significantly lower than those in the model control group( P 0. 05). Conclusion In HSV-1 infected microglia cellular model,CGA inhibits the inflammatory reaction via suppressing TLR2,Myd88,IL-6 in TLR2 signal pathway and inhibiting IL-6 release.
【CateGory Index】： R752.12