Simultaneous Determination of Four Components in Codonopsis Radix Colla Corii Asini Pill by RP-HPLC
LIU Ting;HE Kaiyong;LAN Jing;SONG Danqing;Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences and Peking Union Medical College;National Institutes for Food and Drug Control;Hubei Institute for Drug Control;Beijing Xinlan Pharmatech Co.,Ltd;
Objective To establish an RP-HPLC method forsimultaneous determination of ferulic acid,calycosin-7-glucosid,liquiritin and glycyrrhizic acid in Codonopsis Radix Colla Corii Asini Pill( CCP). Methods The experiments were performed on a Inert Sustain C18 column( 4.6 mm× 250 mm,5 μm) with a gradient mobile phase of 0.5% formic acid( A) and acetonitrile( B).The detection wavelength was 237 nm,the column temperature was 30 ℃,the flow rate was 1.0 m L·min-1 and the injection volume was 20 μL. Results The 4 constituents showed a good linear relationship within the range of of 1. 25-62.47 μg·mL~(-1)( r = 1. 0000) for ferulic acid,4. 84-241. 85 μg · mL~(-1)( r = 1. 0000) for calycosin-7-glucosid,1. 95-97.66 μg·mL~(-1)( r= 0.9999) for liquiritin,18.82-941.16 μg·mL~(-1)( r= 0.9999) for glycyrrhizic acid.The average recovery was103.5%,88.73%,93.77%,100. 2% with the RSDs of 5. 9%,9. 5%,7. 8% and 9. 5%( n = 6),respectively. Conclusion The experiment establishes an RP-HPLC method for simultaneous determination of ferulic acid,calycosin-7-glucosid,liquiritin and glycyrrhizic acid in CCP.It also provides a simple and effective methodto further revise the quality standard of CCP.
【CateGory Index】： R286.0;O657.72