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《China Pharmaceuticals》 2002-01
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Determination of Gentiopicroside i n Compound Capsules of Ursine Gall For Hepatitis B by RP -HPLC

Hu Xiaowei 1 ,Li Yafang 2 (1.Hangzhou Institute For Drug Contr ol,Hangzhou,Zhejiang,China 310014;2.The First People' s Hospital of Hangzhou,Hangzhou,Zhejiang,Chin a 310006)  
Objective:To establish the method to determine the content of Gentiopicroside in Co mpound Capsules of Ursine Gall For Hepatitis B.Methods:Reverse HPLC was applied.The separa tion was performed on Kromasil C 18 column with methol:0.05mol /L KH 2 PO 4 (28∶72)as a mobile phase,and the wavelength of UV detector was 240nm.Results:The linear relationship between con centration and peak area was fine.The average recovery o f Gentiopicroside was 97.06%(RSD =2.33%).Conclusion:The method is simple and reliable,an d also shows good separation.lt can be applied to quality control of Compou nd Capsules of Ursine Gall For Hepati tis B.
【CateGory Index】: R927.2
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