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Determination of Strychnine and Brucine in the Preparation of Jiufen Spraying by RP-HPLC

Sun Yiqun,Huang Yuechun,Xi Ping (The First Hospital of Guangzhou University of TCM,Guangzhou,Guangdong,China 510405)  
Objective:To establish the RP-HPLC method for determination of strychnine and brucine in the preparation of Jiufen Spraying.Methods:RP-HPLC Column was NUCLEODUR C18 (5 μm,4.6 mm×250 mm).Mobile phase was Methanol-Water(65 ∶35)(1 g Sodium dodecyl sulfonate and 0.4 g KH2PO4 in 1 000 mL Water).The detection wavelength was at 254 nm.Results:Strychnine and brucine had a good linearity (r=0.999 5,0.999 2)in the range of 0.174 7~0.873 6 μg and 0.108 8~0.544 4 μg.The mean recovery were 98.92%(RSD=1.70%)and 99.43%(RSD=2.58%).Conclusion:This method is suitable for quality control of this preparation.
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