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《China Pharmaceuticals》 2018-18
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Content Determination of Phlorizin and Trilobatin in Lithocarpus Litseifolius by HPLC

Zhang Yazhou;Wang Tao;Zhu Jingjing;Liu Xue;Zhou Fucai;Wang Qiang;College of Food and Pharmaceutical Engineering,Guizhou Institute of Technology;The Second Affiliated Hospital of Guiyang University of Chinese Medicine;Guangxi Ganliangjian Biotechnology Co.,Ltd.;  
Objective To determine the content of phlorizin and trilobatin in 15 samples of Lithocarpus litseifolius,and to compare their differences. Methods HPLC method was adopted,the chromatographic column was Phenomenex Gemini C18 column( 250 mm × 4. 6 mm,5 μm),the detection wavelength was 280 nm,the mobile phase was acetonitrile-water( 0. 1% phosphoric acid),gradient elution,the flow rate was 1. 0 m L/min. Results The concentration of phlorizin in the range of 0. 250-5. 000 μg showed a good correlation,and the regression equation was C = 0. 000 603 A-0. 021,r = 0. 999 8( n = 7). The concentration of trilobatin in the range of 0. 575-5. 750 μg showed a good correlation,and the regression equation was C = 0. 000 470 A-0. 041,r = 0. 999 6( n = 7). The content of phlorizin in15 samples was in the range of 0. 62%-9. 04%,and trilobatin was in the range of 0. 30%-22. 79%. Conclusion The method is simple and accurate,which can be used for the content determination of phlorizin and trilobatin in Lithocarpus litseifolius.
【Fund】: 国家自然科学基金[81760857];; 贵州理工学院博士启动基金[XJGC20141106]
【CateGory Index】: R284.1
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