Antitumor Activity and Pharmacological Mechanism of Compound Dichloroacetic Acid Diisopropylamine on Esophageal Cancer
Song Zhumei;Song Hongmei;Gui Jinchuan;Wu Haibo;Dazhou Central Hospital;
Objective To investigate the antitumor activity and pharmacological mechanism of compound dichloroacetic acid diisopropylamine( DADA) on esophageal cancer. Methods The esophageal squamous carcinoma cell lines Eca-109 and TE-13 were purchased for the subcutaneous tumor-bearing models of rats. The expression of γ-H2 AX,cell cycle,apoptosis and ROS levels in cell lines of esophageal cancer were detected by staining and flow cytometry,Western blot detection method,and reactive oxygen species( ROS)detection kit. Results The antitumor activity of DADA on Eca-109 and TE-13 cell lines was higher than that of DCA. The number of cells passing through the ventricular membranes of DADA-treated Eca-109 and TE-13 cell lines were significantly reduced,the content of Cleaved Caspase-3 protein in cells were increased significantly( P 0. 05). Compared with radiotherapy alone,the sensitivity of esophageal squamous cell carcinoma Eca-109 and TE-13 cell lines increased by 1. 38 times and 1. 05 times respectively afterDADA combined with radiotherapy. Compared with DADA group and radiotherapy group,DADA combined with radiotherapy group showed that the levels of γ-H2 AX protein in the Eca-109 and TE-13 cell lines were significantly increased,the number of cells in G2/M cycle was significantly increased and the level of ROS was significantly increased( P 0. 05). The results of in vivo validation showed that the tumor volume and weight of DADA combined with radiotherapy were significantly lower than those of DADA or radiotherapy alone( P 0. 05). Conclusion DADA can inhibit the growth of esophageal cancer cell,promote apoptosis and inhibit its migration,combined with radiotherapy can increase the ROS level in cancer cells,and increase the sensitivity of esophageal cancer to radiotherapy,so as to better exert its antitumor activity.
【CateGory Index】： R965