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《China Pharmaceuticals》 2018-18
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Identification and Limit Test of Rumohuoluo San

Chen Siying;Tian Yong;Li Lina;Lu Yuan;Wang Yonglin;Li Yongjun;Wang Aimin;Guizhou Provincial Key Laboratory of Pharmaceutics,State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University;Sinan Tianshi Traditional Orthopedics and Traumatology Hospital;School of Pharmacy,Guizhou Medical University;Engineering Research Center for the Development and Application of Ethnic Medicine and TCM < Ministry of Education>,Guizhou Medical University;  
Objective To establish methods of microscopic identification,TLC identification and limit test of diester alkaloid for Rumohuoluo San. Methods The appearance and properties of the components of Rumeihuoluo San were identified by microscopic identification,and the three kinds of herbs in Rumeihuoluo San were qualitatively identified by TLC,and the amount of diester alkaloids in the preparation was carried out limit test. Results Six herbs in Rumohuoluo San were identified by microscopical identification method,including Panax notoginseng,Boswellia carterii and Commiphora myrrha,ect. Boswellia carterii,Caesalpinia sappan and Dipsacus asper were identified by TLC. The spots were obvious,the chromatographic bands were clear,and the negative controls had no interference. The limit concentration of diester alkaloid in Rumohuoluo San could be detected by TLC. Conclusion The established identification method and inspection method were stable and reliable,which can be used for the quality control of Rumohuoluo San.
【Fund】: 贵州省优秀青年科技人才培养对象专项资金[黔科合人字(2015)11号];; 贵州省科技计划项目[黔科合平台人才(2016)5613;黔科合平台人才(2016)5677]
【CateGory Index】: R286.0
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