Determination of Saikosaponin a and d Content in Spring Bupleurum
ZHU Ming-heng;Pharmacy Department of Suzhou Hospital Affiliated to Nanjing Medical University;
Objective: To determine the saikosaponin a and d content in spring Bupleurum. Methods: HPLC was used. Column was Diamonsil C18(4.6 mm × 250 mm,5 μm),mobile phase was acetonitrile- water gradient elution,1mL·min- 1of flow velocity and210 nm of detection wavelength. Results: The saikosaponin a had a good linear relationship at range of 0. 4018 ~ 6. 027 μg(r = 0. 9990).The average recovery rate was 91. 19% and RSD was 1. 83%( n = 6); the saikosaponin d had a good linear relationship at range of0. 5238 ~ 7. 857μg(r 0. 9992). The average recovery rate was 92. 76% and RSD was 1. 43%(n = 6). Conclusion: The study establishes a determination method for saikosaponin a and d,which is simple and accurate.
【CateGory Index】： R284.1