Research on Measurement of Combined Antibodies in the Skin Tissue in Auto-immune Diseases by HRP-SPA Technique
Sun Guowen, et al Department of Dermatology, First Affiliated Hospital, Chinese Medical university
The authors inspected the conjugatedantibodies in the cutaneous tissues of 23 patients with lupus erythematosus,pemph igus and bullous pemphigoid by the technieque of Horseradish Peroxidaselabeled Staphylococcal Protien A(HRP-SPA).There were positive results in all cases. The regions of these depositions were respectively seen in the dermal-epidermal conjunction, inter-prickle cells and subepidermal basement membrane. As the method has the advantages of simple operation, longer conservation time of biopsy specimens, and can be especially done in the laboratory without fluorescent microscope, it is worth recommending.