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The Effect of Mechlorethamine on Cytokines Secretion of Human Keratinocyte HaCaT Cell Line in Vitro

GU Jun,Zhou Chun-lei (Department of Dermatology,Changhai Hospital,Second Military Medical University,Shanghai 200433,China)  
Objective To Study the effect of mechlorethamine on cytokines secretion of human keratinocyte HaCaT cell line in vitro,both not stimulated and stimulated by PMA/LPS.Methods Expression of IL-1α,IL-2,IL-6 and IL-8 was detected by ELISA kit.Results HaCaT cell secreted IL-1α,IL-2,IL-6 and IL-8 spontaneously;Stimulation by PMA/LPS induced significant increase in IL-6 and IL-8 level and decrease in IL-1α level in HaCaT cell,a pattern similar to those seen in psoriasis.On HaCaT cell,Mechlorethamine increased the secretion of IL-1α,IL-6 and IL-8 distinctly,they may participate in the inflammation of Mechlorethamine on normal skin.On HaCaT cell stimulated with PMA/LPS,Mechlorethamine decreased the secretion of IL-6,IL-8 and increased the secretion of IL-1α in a low concentration,but the secretion of IL-6,IL-8 and IL-1α in stimulated HaCaT cell were significantly increased when Mechlorethamine was in a high concentration.Conclusion These results suggested that the mechanism of Mechlorethamine therapy on psoriasis is related to IL-1α,IL-6 and IL-8.
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