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Analysis of Specific Antibodies During Treponema Pallidum Infection and Treatment in Rabbit

DANG Qian-li,LU Xue-dong,ZHANG Xiao-yan, et al (Department of Dermatology Shaanxi Provincial People’s Hospital,Xi’an Jiaotong University,Xi’an 710068,China)  
Objective To detect antibodies to treponema pallidum for the early accurate diagnosis of syphilis and direction of clinical treatment.Methods The model of rabbit’s syphilis was founded.By using RPR,TPPA and western immunoblotting technique (WIB),the change of antibodies to treponema pallidum after infection and treatment was tested. Results A positive antibody of molecular 47kD was detected in serum infected rabbit in 3 days by using WIB,and then the specificities of 45kD,17kD,15.5kD,and 92kD-protein antibody were detected.Having been finished treatment,45kD,15.5kD, and 92kD protein antibodies of some rabbits were gradually weak and disappear.Using RPR for syphilis,positive serologic phosphatidic antibodies titers were gradually reduced with treatment in 1-1.5 month after infection.Positive serologic antibodies were later detected by using TPPA than by WIB,Fastigium and change of time of TP antibodies was later than phosphatidic antibodies.Conclusion This study demonstrates that 47kD serum antibodies detected by WIB could be used for the early accurate serum test diagnosis of syphilis;the change of 45kD,15.5kD,and 92kD protein antibodies by using WIB and the change of positive serologic titers by using RPR may be direction of clinical treatment for syphilis.
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