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《The Chinese Journal of Dermatovenereology》 2013-01
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Detection TP53 Protein Expression and Gene Mutation in Langerhans’Cell Histiocytosis

SUN Zhong-hui1, SHI Mei2, LI Ming2, YU Hong2 (1. Department of Dermatology,Fengxian Institute of Dermotosis Prevention,Shanghai 201408,China; 2. Department of Dermatology,Xinhua Hospital of Shanghai Jiaotong University,Shanghai 200092,China)  
Objective To determine whether TP53 gene plays a role in the pathogenesis of Langerhans’ Cell Histiocytosis (LCH),we studied the expression of TP53 protein and mutation of TP53 gene in LCH biopsies. Methods The expression of TP53 protein was detected by immunohistochemical SP method in 38 cases of Langerhans cell histiocytosis. The mutation in TP53 gene exon 2 to 11 was identified by polymerase chain reaction and subsequently conformed by DNA sequencing in the 16 TP53 protein positive biopsies. Results TP53 protein expression was detected in 16/38(42.11%) LCH biopsies. In those biopsies,there were no significant difference between the average optical density of multisystem (MS,0.120 6±0.008 8) and single-system (SS,0.122 1±0.003 0) respectively(P0.05). No mutations in the TP53 gene exon 2 to 11 were detected in those positive biopsies. For genetic genotype of the polymorphism of TP53 gene codon 72,there were PRO in 3 cases,ARG in 5 cases, PRO/ARG in 8 cases in LCH group (TP53 protein positive). Compared with 40 normal controls,Chi-square analysis showed there was no significant difference for distribution of the three genotypes(P0.05).Then,for the clinical classification,the cases of PRO,ARG and PRO/ARG were 2,3,5 in MS and 1,2,3 in SS,respectively. Chi-square analysis also showed no significant differences (P0.05). Conclusion The TP53 protein is abnormally expressed in LCH,although there was no significant difference with the clinical classification. There is no TP53 gene mutation in LCH. The TP53 codon 72 genotype distribution was consistent with the normal population and not related with clinical classification. We speculate that the abnormality of TP53 gene expression in LCH may not result from gene mutation but an unknown mechanism.
【Fund】: 上海市卫生局项目(NO.2009065)
【CateGory Index】: R758.6
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