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Determination of geniposide in Qinghuo Zhimai Tablets by HPLC-Densitometry

Liu Huagang; Chen Yanjun and Mao Jian (Guangxi College of Traditional Chinese Medicine, Nanning 530001)  
Content of geniposide in Qinghuo Zhimai tablet(QZM) was determined by RP-HPLC with the use of external standard. Conditions for the extraction and determination of geniposide from QZM were optimized. Results showed that the samples should be washed with ethyl ether to remove fatty impurities and then extracted with 50% methanol with the aid of ultrasonic wave. Samples from five different manufacturers were determined. Their geniposide contents varied from 0.356 mg/Tab, 0.467 mg/Tab, 0.957 mg/Tab,2.975 mg/Tab and 5.387 mg/Tab. The average recovery was 97.4% and RSD=1.61%.
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