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A Study on the Tissue Culture and Plant Regeneration of Trifoliate-orange (Poncirus trifoliata)

He Hong (Institute of Chinese Materia Medica, Guangzhou University of Traditional Chinese Medicine and Materia Medica, Guangzhou 510407)  
Epicotyls from sterile seedling of Poncirus trifoliata (L.)Raf. were cultured in vitro. Results indicated that BA at a concentration of 1 mg/L gave the highest shoot formation frequency (86.0%).As BA concentration increased, the ability of shoot formation decreased. The capacity of shoot regeneration was also related to the age of sterile seedling, the explants from 20 days sterile seedling were optimal. There was no significant difference on shoot formation of the explants from sterile seedling that was cultured under light or in dark. When epicotyls from different part of sterile seedling were cultured, the highest shoot induction frequency (over 95%) was obtained from the explants excised from middle and basal end, and the shoot induction frequency of the apical end explants decreased markedly
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