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《Chinese Traditional and Herbal Drugs》 2006-12
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Inducement of ginsenoside Rg_1 on expression of glucocorticoid receptor report gene in HL-7702 cells

LI Yong1, LI Yi-ping1, YU Sheng, LING Chang-quan2(1.Shanghai Hospital of Traditional Chinese Medicine Affiliateed in Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China; 2. Department of Traditional Chinese Medicine, The Second Military Medical University, Shanghai 200433, China)  
Objective To investigate the effect of ginsenoside Rg1 (G-Rg1) on regulation of glucocorticoid receptor (GR) in HL-7702 cells. Methods HL-7702 Cells were transiently cotransfected by Lipofect AMINETM2000 with pGRE-tk-LUC reporter plasmid and pRL-SV40 plasmid. The effects of G-Rg1 and Dexamethasone on luciferase activity expression were detected by Dual-Luciferase Reporter Assays, the protein expression of GR in HL-7702 cells was analyzed by Western blotting assay, respectively. Results A dose-dependent induction of the reporter gene was observed in response to Dex or G-Rg1, and the inductive effect was blocked by treatment with the antiglucocorticoid RU486 (Mifepristone). G-Rg1 also down-regulated the GR protein expression in HL-7702 cells. ConclusionThese results strongly indicate that G-Rg1, which may be a ligand of GR, has some glucocorticoid-like effect in vitro.
【Fund】: 国家自然科学基金资助项目(30472271)
【CateGory Index】: R285.5
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