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《Chinese Traditional and Herbal Drugs》 2007-01
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Total saponins of Panax ginseng inducing CD34~+ cells to differentiate into granulocytopoietic lineage in vitro

WANG Jian-wei,WANG Ya-ping,WANG Sha-li,JIANG Rong(Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology,School of Basic Medicine,Chongqing University of Medical Sciences,Chongqing 400016,China)  
Objective To investigate the effect of recombinant between hematopoietic growth factors and total saponins of Panax ginseng(TSPG)on granulocytopoietic differentiation of purified human CD34+ cells derived from human bone marrow cells.Methods A sterilized separation system for purification of CD34+ hematopoietic stem/progentior cells(HSC/HPC)was established by means of StemsepTM according to the strategy of negative selection on immunomagnetic separation.The capacity of various concentrations of TSPG inducing CD34+ cells to differentiate into CFU-GM cells was detected by methyl cellulose assay systems;CD34+ cells were committedly differentiated with various combination of cytokines in liquid culture systems and the expanded cell number,CD33+ cell ratio and granulocytopoietic progenitor cell number were detected;various concentrations of TSPG were then added into liquid culture systems containging SCF+IL-3+GM-CSF+G-CSF(SIGG),the elevation rate on proliferation of CD34+ cells and the formation of CFU-GM were observed and the ratio of CD33+ cells were estimated.ResultsTSPG(10—50 μg/mL)could increase the colony yield of CFU-GM,TSPG(20 μg/mL)did best.At the various concentrations of TSPG synergized with hematopoietic growth factors on granulocytopoietic differentiation of CD34+ cells for two weeks in liquid culture systems,TSPG(10—70 μg/mL)could increase the expanded cell number,the amplification multiple of total CFU-GM,and CD33+ cell ratio;TSPG 20 μg/mL was identified as the most potential concentration for the granulocytopoietic differentiation of CD34+ cells in liquid culture system.Conclusion TSPG may promote CD34+ cells to proliferate and differentiate to granulocytopoietic lineage by synergism with hematopoietic growth factors.
【Fund】: 国家自然科学基金资助项目(编号38670880 30472253)
【CateGory Index】: R285.5
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【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Qiang 1,WANG Bing 2, LI He lian 1,WANG Wei 3(1.Department of Obstetrics and Gynecology, Second Hospital,Jilin University,Changchun 130041,China;2. Department of Plastic Surgery,China Japan Union Hospital,Jilin University, Changchun 130031,China; 3. Department of Rehabilitation,First Hospital,Jilin University, Changchun 130021,China);Effects of many kinds of cytokines on CD34~+ cell proliferation and differentiation[J];Journal of Jilin University of (Medicine Edition);2004-02
2 ;The progress in the Basis and Application of Stem Cells[J];Acta Academiae Medicinae Militaris Tertiae;2003-01
3 QIU Guang,WU Xiao-qiong,LUO Xue-gang~*(Department of Anatomy and Neurobiology,Xiangya School of Medicine,Central South University,Changsha 410013,China;Effect of polygonum multiflorum thunb on BDNF expression in rat hippocampus induced by amyloid beta-protein(Aβ) 1-40[J];Journal of Central South University(Medical Sciences);2006-02
4 LIU jing-zheng,WANG Qian,ZHENG Lei,et al(Department,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China);Immunomagnetic Separation of CD34~+ Cells from Umbilical Cord Blood and Its[J];Journal of Clinical Research;2008-03
5 WANG Sha-li, LI Jing, WANG Ya-ping *,CHEN Di, WANG Lu, JIANG Rong(College of Basic Medical Sciences, Chongqing University of Medical Sciences, Chongqing 400016,China);STUDY ON THE EXPRESSION OF HEMATOPOIETIC GROWTH FACTORS IN BONE MARROW MARCROPHAGE TREATED WITH SAPONINS OF PANAX GINSENG[J];Acta Anatomica Sinica;2003-05
6 WANG Lu,WANG Ya-ping * (Department of Histology and Embryology,Chongqing University of Medical Sciences,Chongqing 400016,China);EXPERIMENTAL STUDY FOR THE EFFECT OF TSPG ON THE EXPRESSION OF IL-3 IN HEMATOPOIETIC STROMAL CELLS[J];Acta Anatomica Sinica;2004-01
7 Wang Yaping;Zhu Bide Chongqing University of Medical Sciences,Chongqing 630046;EFFECT OF ANGELICA POLYSAACHARIDE ON GRANULOPOIESIS AND MONOCYTOPOIESIS IN MICE[J];Chinese Journal of Anatomy;1993-02
8 Wang Yong, Wang Yaping, Zhu Bide(Department of Histology and Embryology, Chongqing University of Medical Sciences,Chongqing 630046);EFFECT OF TOTAL SAPONINS OF PANAX GINSENG ON PROLIFERATION OF HEMATOPOIETIC CELLS IN MICE[J];Chinese Journal of Anatomy;1995-02
9 Wang Yaping, Niu Hong Jiang Rang, Wang Yong, Zhu Bide (Chongqing University of Medical Sciences, Chongqing 630046);THE EFFECT OF TOTAL SAPONINS OF PANAX NOTOGINSENG ON PROLIFERATION OF ERYTHROID PROGENITORS AND ITS MECHANISM[J];Chinese Journal of Anatomy;1996-02
10 ZHENG Min, WANG Ya Ping (Institute of Basic Medical Science, Chongqing, University of Medical Sciences, Chongqing 400016);Study on biological mechanism of angelica polysaccharide regulation on human early multipotential progenitor cell[J];Chinese Journal of Anatomy;2002-02
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