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《Chinese Traditional and Herbal Drugs》 2007-01
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Total saponins of Panax ginseng inducing CD34~+ cells to differentiate into granulocytopoietic lineage in vitro

WANG Jian-wei,WANG Ya-ping,WANG Sha-li,JIANG Rong(Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology,School of Basic Medicine,Chongqing University of Medical Sciences,Chongqing 400016,China)  
Objective To investigate the effect of recombinant between hematopoietic growth factors and total saponins of Panax ginseng(TSPG)on granulocytopoietic differentiation of purified human CD34+ cells derived from human bone marrow cells.Methods A sterilized separation system for purification of CD34+ hematopoietic stem/progentior cells(HSC/HPC)was established by means of StemsepTM according to the strategy of negative selection on immunomagnetic separation.The capacity of various concentrations of TSPG inducing CD34+ cells to differentiate into CFU-GM cells was detected by methyl cellulose assay systems;CD34+ cells were committedly differentiated with various combination of cytokines in liquid culture systems and the expanded cell number,CD33+ cell ratio and granulocytopoietic progenitor cell number were detected;various concentrations of TSPG were then added into liquid culture systems containging SCF+IL-3+GM-CSF+G-CSF(SIGG),the elevation rate on proliferation of CD34+ cells and the formation of CFU-GM were observed and the ratio of CD33+ cells were estimated.ResultsTSPG(10—50 μg/mL)could increase the colony yield of CFU-GM,TSPG(20 μg/mL)did best.At the various concentrations of TSPG synergized with hematopoietic growth factors on granulocytopoietic differentiation of CD34+ cells for two weeks in liquid culture systems,TSPG(10—70 μg/mL)could increase the expanded cell number,the amplification multiple of total CFU-GM,and CD33+ cell ratio;TSPG 20 μg/mL was identified as the most potential concentration for the granulocytopoietic differentiation of CD34+ cells in liquid culture system.Conclusion TSPG may promote CD34+ cells to proliferate and differentiate to granulocytopoietic lineage by synergism with hematopoietic growth factors.
【Fund】: 国家自然科学基金资助项目(编号38670880 30472253)
【CateGory Index】: R285.5
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Chinese Journal Full-text Database 10 Hits
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