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Effect of tetramethylpyrazine on hypoxic model in human umbilical vein endothelium cells and its functions of anticoagulation and fibrinolysis

ZHANG Guo-ping, SUN Mei, YANG Jie-hong, WAN Hai-tong(1.Department of Traditional Chinese Medicine, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China; 2. Institute of Cardio-cerebral Diseases, Zhejiang College of Traditional Chinese Medicine, Hangzhou 310053, China)  
Objective To set the hypoxic model in human umbilical vein endothelium cells (HUVECs) and observe the effect of tetramethylpyrazine in its functions of anticoagulation and fibrinolysis. MethodsPreparing the normal primary HUVECs, when the cultured endothelial cells (ECs) were inosculated they were put into the anoxic condition with 95% N2 and 5% CO2 to make hypoxic model in primary HUVECs. Normal, model, and tetramethylpyrazine groups were divided and used to observe the difference of floating cells, anticoagulation and fibrinolysis indexes in supernatant of each group. Results Compared with the control group, the number of floating cells in supernatant was increased obviously, the level of 6-keto-PGF1α was declined significantly, the activity of plasminogen activator inhibitor (PAI) and the level of endothelin (ET) were enhanced obviously, the activity of tissue plasminogen activator (t-PA) and the level of NO were declined significantly in model group; the level of 6-keto-PGF1α was raised significantly, the activity of PAI and t-PA, and the levels of ET and NO could be regulated in the tetramethylpyrazine group. Conclusion In the hypoxic model of primary HUVECs made in this experiment, ECs are injured, secreting function is declined, vasomotion is out of control. Tetramethylpyrazine could protect hypoxic ECs from being injured, regulate the secreting function of ECs and the vasomotion. The model could provide a method for basic study and medicine selection of anticoagulation and fibrinolysis.
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