HPLC Fingerprint of flavonoids from Lycium barbarum
ZHANG Zi-ping1, LIAO Guo-ling1,2, LI Hong-wu1(1. Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resourcesin Western, Ningxia University, Yinchuan 750021, China; 2. Department for Drug Control,Ningxia Medical College, Yinchuan 750004, China)
Objective To establish chromatographic fingerprint of flavonoids from Lycium barbarum by HPLC and distinguish the Fructus Lycii from different habitats. Methods The mutual mode was established depending on ten Fructus Lycii samples from different growing areas in Ningxia. The solftware "Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica" was applied to analyzing 15 Fructus Lycii samples from different habitats. Results The established chromatographic fingerprint method had good repeatability; 16 peaks are characteristic of HPLC fingerprints of flavonoids and flavonoids of Fructus Lycii from different habitats were quite different in content and constituent. Conclusion This chromatographic fingerprint spectrum of flavonoids could be used to distinguish Fructus Lycii from different habitats.