Isolation and structure identification of steroidal saponin from Dioscorea zingiberensis
CHENG Juan1,HU Chang-ying2,PANG Zi-jie3,XU De-ping1(1. School of Food Science and Technology,Southern Yangtze University,Wuxi 214122,China;2. Depertment of Food Science and Engineering,Jinan University,Guangzhou 510520,China;3. Institute of Botany,Jiangsu Province and Chinese Academy of Science,Nanjing 210014,China)
Objective To study the chemical constituents of Dioscorea zingiberensis.Methods The fresh rhizome of D.zingiberensis was extracted three times,3 h once with EtOH-H2O at 80 ℃.The EtOH was evaporated under reduced pressure to give a residue which was suspended in water and then was exacted with petroleum ether,ethyl acetate,and n-butanol fraction.The water fraction was isolated by the reversed-phase ODS column chromatography.The chemical structures were elucidated by means of 1H-NMR,13C-NMR,135DEPT,HMQC,and HMBC spectroscopic analyses.Results Three steroidal saponins were isolated from the fresh rhizome of D.zingiberensis.The compounds were identified as:diosgenin-3-O-β-D-glucopyranosyl(1→3)-β-D-glucopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-glucopyranoside(Ⅰ),(25R)-26-O-β-D-glucopyranosyl-furost-5-en-3β,22ζ-diol-3-O-β-D-glucopyranosyl(1→3)-β-D-glucopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-glucopyranoside(Ⅱ),and(25R)-26-O-β-D-glucopyranosyl-furost-5-en-3β,22ζ-diol-7-carbonyl-3-O-β-D-glucopyranosyl(1→3)-β-D-glucopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-glucopyranoside(Ⅲ).Conclusion Compound Ⅲ is a novel compound named as zingiberenin H.
【CateGory Index】： R284.1