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《Chinese Traditional and Herbal Drugs》 2009-01
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Studies on HPLC fingerprint of Herba Rhodobryi Rosei

CAI Ying1,2,LU Xiao-he1,WEI Qun-li1,CHEN Rui-hua1(1.Nanjing General Hospital of Nanjing Military Command,Nanjing 210002,China;2.No.454 Hospital of PLA,Nanjing 210002,China)  
Objective To establish an HPLC fingerprint of Herba Rhodobryi Rosei.Methods The fingerprint chromatography has been determined by RP-HPLC.The analysis was carried out with Dikma ODS C18 column(250 mm×4.6 mm,5 μm).The mobile phase were:0.5% HAcCN(A),0.5% HAcH2O(B);Elution method:0-50 min,A was 20%-55%;50-60 min,A was 55%-95%;60-85 min,A was 95%-100%;keeping 5 min.Flow-rate was 1.0 mL/min.Wavelength was 360 nm and temperature was 30 ℃.It was analysized with the Estimating System of Similarity of 2004A Version(the Country's Pharmacopeia Committee)on the Chinese Medicine Fingerprint Chromatography.Results The fingerprint chromatography of Herba Rhodobryi Rosei was estabilished.Conclusion The method can be used in quality control of Herba Rhodobryi Rosei with accuracy and better repeatability.
【CateGory Index】: R284
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