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《Chinese Traditional and Herbal Drugs》 2012-01
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Spectrum-activity relationship of hemagglutination components in Isatidis Radix

SUN Qin1,MA Li2,LI Lan1,HU Xiao-yan1,JIANG Li1,XIAO Xiao-he3 1.Department of Pharmacy,Luzhou Medical College,Luzhou 646000,China 2.School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China 3.PLA Institute of Chinese Materia Medica,302 Hospital of PLA,Beijing 100039,China  
Objective To study the active components in Isatidis Radix with agglutination effect on red blood cells.Methods The different extracts from Isatidis Radix were obtained by the means of systematic solvent extraction and their antivirus activity was studied by hemagglutination test.The HPLC fingerprints of the most active extracts from Isatidis Radix were established and also the extracts were bioassayed.The spectrum-activity relationship of the extracts was studied by mathematical statistics method.Results The n-butanol extracts had the best agglutination effect on red blood cells of the rabbit.The study on the spectrum-activity relationship of the extracts found that the greater the proportional area of common peaks 2 and 11(retention time were 7.23 and 43.00 min) of the extracts had,the stronger the agglutination effect of the extracts had.Conclusion Common peaks 2 and 11 could be characteristic peak for anti-virus activity of Isatidis Radix.The spectrum-activity relationship of Isatidis Radix established could provide the theoretical basis and the data support for the pattern of quality control of Chinese materia medica.
【Fund】: 国家自然科学基金资助项目(30873381 30701091)
【CateGory Index】: R284.2
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