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《Chinese Traditional and Herbal Drugs》 2013-01
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Comparative analysis of chemical constituents in different parts of Siraitia grosvenorii using UPLC-MS combined with pattern recognition

ZHANG Hong-yang 1,YANG Hui-hua 2,ZHANG Min 3,WANG Yue-rong 1,WANG Jing-rong 4,JIANG Zhi-hong 4,HU Ping 1 1.School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai 200237,China 2.College of Computer Science and Control,Guilin University of Electronic Technology,Guilin 541004,China 3.Modern Engineering Center for Traditional Chinese Medicine,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China 4.State Key Laboratory for Quality Research in Chinese Medicines,Macau University of Science and Technology,Macau,China  
Objective To develop an ultra performance liquid chromatography/time-of-flight mass spectrometry(UPLC/TOF-MS) method for the comparative analysis of chemical constituents in the different parts(pulp,pericarp,leaf,and stem) of Siraitia grosvenorii.Methods Chromatographic separation was performed on the Waters Acquity HSS C18column(100 mm × 2.1 mm,1.7 μm) and eluted by gradient way with the mobile phase of 0.1% formic acid in water-acetonitrile.Electrospray ionization source was used for the mass spectrometric analysis under negative ion mode.The obtained data were then analyzed via pattern recognition using principle component analysis(PCA).Results The PCA indicated that the constituents existed in the pulp and pericarp were similar,which were significantly different from those in the leaf and stem.Thirteen characteristic compounds accounting for such variations were screened as "markers".TOF-MS was used for accurating molecular mass,isotopic matching,and chromatographic retention regulation,five mogrosides(markers of fruit) and five flavonol glycosides(markers of leaf and stem) were identified.Conclusion This research could provide a novel pattern for the identification and comparison of chemical constituents in the different parts of S.grosvenorii and the scientific information for the study on its active components.
【Fund】: 国家自然科学基金资助项目(30860381和21006023)
【CateGory Index】: R284.1
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