Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Traditional and Herbal Drugs》 2013-01
Add to Favorite Get Latest Update

Effects of mangiferin on MAPK pathway and serum cytokines in rats with chronic inflammation induced by lipopolysaccharide

WEI Zhi-quan1,YAN Li1,DENG Jia-gang1,DENG Jing2 1.Guangxi University of Chinese Medicine,Nanning 530001,China 2.Dana-Farber Cancer Institute,Harvard Medical School,Boston 02115,USA  
Objective To investigate the regulation of mangiferin on mitogen-activated protein kinases(MAPK) pathway and the serum cytokines expression patterns in chronic lipopolysaccharide(LPS)-induced chronic inflammation of rats.Methods The rat model with chronic inflammation was established by iv low dose(200 μg/kg) intermittent LPS injection via the caudal vein.Rats were randomly divided into model,Prednisone(PNS,5 mg/kg),and mangiferin high-,mid-,low-dose(200,100,and 50 mg/kg) groups.The rats were ig administered with the predetermined drugs once a day within four weeks.At the end of the 4th week,the RT-PCR was applied to evaluate the gene expression of p38,ERK,and JNK in the MAPK pathway,while the Western blotting was executed to detect their protein phosphorylation levels.The protein antibody microarray chip was employed for serum cytokine expression pattern measurement.Results Compared with the model group,both of the expression of ERK,JNK,and the protein phosphorylation of ERK were markedly inhibited in mangiferin high-dose group.The levels of intercellular adhesion molecule 1(ICAM-1),monocyte chemoattractant protein 1(MCP-1),cytokine-induced neutrophil chemoattractant 1(INC-1),and platelet-derived growth factor(PDGF) were significantly lowered.Conclusion Mangiferin could simultaneously regulate the gene expression of ERK,JNK,and the protein phosphorylation of ERK in the MAPK pathway and decrease the serum chemotactic cytokines level.
【Fund】: 国家自然科学基金资助项目(30973916);; 广西科技厅中药药效研究开放实验室资助项目(10-046-04-Z11)
【CateGory Index】: R285.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved