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《Chinese Traditional and Herbal Drugs》 2013-15
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Identification of Zanthoxyli Radix and its adulterants using ITS2 barcoding

CHEN Bei-bei 1,SONG Jing-yuan 2,YAO Hui 2,HAN Zheng-zhou 1,YANG Tie-chui 1,XING Jian-yong 1 1.Research Center,China Resources of Sanjiu Medical & Pharmaceutical Co.,Ltd.,Shenzhen 518110,China 2.Institute of Medicinal Plant Development,Chinese Academy of Medical Science & Peking Union Medical College,Beijing 100193,China  
Objective ITS2 barcoding was used to discriminate Zanthoxyli Radix and its adulterants to ensure the quality and clinical safety of this Chinese materia medica.Methods The internal transcribed spacer 2(ITS2) regions were amplified and sequenced bi-directionally.Then the obtained sequences were assembled using the CodonCode Aligner.The ITS2 regions were obtained using the hidden Markov model(HMM)-based annotation methods.The genetic distances of the ITS2 regions were computed in accordance with the kimura 2-parameter(K2P) model and Neighbor-joining(NJ) phylogenetic trees were constructed using MEGA5.0.Results The length of ITS2 sequences of the plants in Zanthoxyli Radix and its adulterants were between 224 and 227 bp.Their mean intraspecific genetic distance(K2P distance) was lower than their mean interspecific genetic distance with the adulterants.The NJ trees showed that the roots of Zanthoxyli Radix could be easily distinguished from its adulterants.Conclusion ITS2 barcode could be used to identify the roots of Zanthoxyli Radix and its adulterants effectively,and provide the important molecular evidence for the authentication of germplasm resources.
【Fund】: 广东省教育部产学研结合项目(2009B090300202)
【CateGory Index】: R282.5
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