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《Chinese Traditional and Herbal Drugs》 2013-23
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Chemical constituents of non-saponin from Pulsatilla cernua

YU Zong-gui;LIU Jian-yu;GONG Yi-xia;FAN Wen-hao;MA Jing;ZHOU Nan;XU Yong-nan;Key Laboratory of Structure-Based Drug Design Discovery,Ministry of Education,School of Pharmaceutical Engineering,Shenyang Pharmaceutical University;  
Objective To isolate and identify the chemical constituents from the roots of Pulsatilla cernua.Methods Compounds were isolated by various kinds of column chromatographies on silica gel,Sephadex LH-20,and recycling preparative HPLC,and their structures were elucidated by the physicochemical characters and spectral analysis.Results Fifteen chemical constituents were obtained and identified as 5-hydroxy-2,2-dimethyl-2H-chromene-6-carbaldehyde(1),pulsaquinone(2),aurantiamide acetate(3),stigmasterol(4),hexadecanoic acid(5),aurantiamide(6),pinoresinol(7),buplerol(8),neoechinulin A(9),(2S,3S,4R,8E)-2-[(2′R)-2′-hydroxypalmitoylamino]-8-octadecene-1,3,4-triol(10),5R-dihydro-5-hydroxymethyl-2(3H)-furanone(11),caffeic acid(12),methyl 6-dehydroxyl rosmarinate(13),hederagonic acid(14),and 5-hydroxy-6,8-dimethoxycoumarin(15),respectively.Conclusion Compound 1 is a new natural product,compounds 3,6,8—10,13,and 15 are isolated from the plants in Pulsatilla Adans.for the first time,and compound 7 is isolated from this plant for the first time.
【CateGory Index】: R284.1
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