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《Chinese Traditional and Herbal Drugs》 2013-23
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Study of microbial transformation process of Strychnos nux-vomica alkaloid salts by Cunninghamella blakesleeana

PAN Zi-hao;JIN Miao;PAN Yang;College of Pharmacy,Nanjing University of Chinese Medicine;Nanjing Institute of Geography & Limnology,Chinese Academy of Sciences;  
Objective To enhance the transformation rate of brucine and strychnine in Strychnos nux-vomica and establish a high efficient microbial transformation system by Cunninghamella blakesleeana.Methods Based on the growth and metabolic rule of C.blakesleeana,through single-factor experiments,by using brucine sulphate and strychnine nitrate as substrates and the transformation rate of each substrate as index,several influential factors were investigated.Results The optimal transformation condition was inoculation amount at 4%,fermentation time at 2.5 d,substrate concentration at 20 mg/L,transformation time at 3 d,culture temperature at 28 ℃,initial pH of medium at 6.5,and shaking speed at 150 r/min.Under the above condition,the average transformation rates of brucine sulphate and strychnine nitrate were approximate 77.75% and 77.10%,respectively.Conclusion The average transformation rates of brucine sulphate and strychnine nitrate are increased by about 17% and 22%,respectively by C.blakesleeana,and this microbial transformation system could meet the need of the study on "toxicity attenuation and efficacy reservation" of S.nux-vomica.
【Fund】: 江苏省高校优势学科建设工程资助项目(ysxk-2010);; 南京中医药大学青年自然科学基金资助项目(11XZR13)
【CateGory Index】: R283
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