Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Traditional and Herbal Drugs》 2013-23
Add to Favorite Get Latest Update

Cloning and differential expression of chalcone synthase gene 1 in Houttuynia cordata and its protein sequence analysis

WEI Lin;WU Xian-jin;LIU Sheng-gui;TANG Yu-lian;HE An-na;XU Jie;WU Cheng-jiu;Key Laboratory of Xiangxi Medicinal Plant and Ethnobotany of Hunan Higher Education,Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province,Department of Life Sciences,Huaihua University;College of Agronomy,Sichuan Agricultural University;Xiangya Medical Laboratory,Central South University;  
Objective To clone and sequence the chalcone synthase1(CHS1) gene from the leaves of Houttuynia cordata.Methods The cloning primers were designed on the basis of the conserved sequences of the cloned CHS gene in other plants.The total RNA was extracted from the leaves of H.cordata.The sequence of CHS gene was cloned by reverse transcription polymerase chain reaction(RT-PCR) and SON-PCR techiniques,and then the gene was ligated with pMD18-T Simple vector.The positive clone was sequenced after the identification of clone by PCR.Results We cloned a fragment of 1 188 bp.The analysis of sequencing indicated that the fragment encoded 395 amino acids and shared the sequence homology of more than 62.3% with CHS gene sequences from other higher plants.Bioinformatic analysis indicated that the CHS amino acids had no signal peptide sequences,but possessed the CHS family's characteristic sequences,RLMMYQQGCFAGGTVLR and GVLFGFGPGL.Relative real-time PCR analysis indicated that CHS1 showed the highest transcript abundance in the flowers,moderate levels in the stems,lower levels in the rhizomes,and the lowest levels in the leaves.Conclusion It is the first report that a novel CHS gene is cloned from H.cordata,which lays a foundation for the effective use of CHS1 gene.
【Fund】: 国家自然科学基金资助项目(30870230);; 湖南省高校创新平台开放基金项目(12K132);; 湖南省科技计划重点项目(2013FJ6090)
【CateGory Index】: R29
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 8 Hits
1 PAN Ying1,CHEN Chen2,WANG Le2,WEI Ya-chao2 1.Department of Pharmacy,Third Hospital of Hebei Medical University,Shijiazhuang 050051,China 2.Department of Pharmacy,Handan Central Hospital,Handan 056001,China;Clinical curative observation of Yuxingcao Gutta in treatment of acute catarrhal conjunctivitis[J];Drugs & Clinic;2013-03
2 Liu Yi1,2, ZHAI XU-guang1,2,WU Fang1,2, DENG Guang-bing1, PAN Zhi-fen1,YU Mao-qun1** (1.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; 2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China);SON-PCR Cloning the Leucine-rich Repeats Region of Cereal Cyst Nematode Resistance Gene and Sequencing[J];Journal of Agricultural Biotechnology;2006-04
3 DENG Hongyuan 1, 2, WANG Minxia 1, 2, SUN Xuewen 1, 2, MA Xinrong 1, CAO Tingting 1, 2 & TAN Hong 1**( 1Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China)( 2Graduate University of the Chinese Academy of Sciences, Beijing 100039, China);An Improved SON-PCR Method for Gene Amplification[J];Chinese Journal of Applied & Environmental Biology;2006-06
4 ZHOU Lin,WANG Yan,and PENG Zhen-hua (Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China);Isolation and Tissue-specific Expression of Chalcone Synthase Gene Ps-CHS1 in Tree Peony[J];Acta Horticulturae Sinica;2010-08
5 LIN Ling,TANG Hao-ru*,CHEN Qing,LU Min,JIA Hui-feng,LI Ying-gai,and ZHANG Xiao-nan (College of Horticulture,Sichuan Agricultural University,Ya’an,Sichuan 625014,China);Cloning and Sequence Analysis of Chalcone Synthase Gene in Ornamental Peach[J];Acta Horticulturae Sinica;2012-03
6 XIE Nan-nan1,QIAO Yong1,ZHAO Jin1,LIU Meng-jun2(1College of Life Science,Agricultural University of Hebei,Baoding 071000,Hebei;2Research Center of Chinese Jujube,Agricultural University of Hebei,Baoding 071001,Hebei);A SON-PCR Technique Suitable to Amplificating the Flanking Regions of Gene of Chinese Jujube[J];Scientia Agricultura Sinica;2011-04
7 XIAO Yan YANG Zu-Jun~* LI Guang-Rong REN Zheng-Long School of Life Science and Technology,University of Electronic Science and Technology of China,Chengdu 610054,China;SON-PCR Fast Cloning and Sequence Analysis of CHS Gene from Rhodiola fastigiata(Hook.f.et Thoms.)S.H.Fu[J];Plant Physiology Communications;2008-01
8 FU Ming,WEI Lin,YU Juan,YU Xiao-lin Key Laboratory of Xiangxi Medicinal Plant and Ethnobotany of Hunan Higher Education,Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province,Department of Life Sciences,Huaihua University,Huaihua 418008,China;cDNA cloning and protein sequence analysis of chalcone synthase gene in leaves of Ampelopsis grossedentata[J];Chinese Traditional and Herbal Drugs;2013-01
Chinese Journal Full-text Database 10 Hits
1 Zhu Zi Chao et al.(College of Agronomy and Biotechnology,Southwest University,Beibei,400716,China);Genetic Effects of Duplication and Disproportionation of Chalcone Synthase Genes[J];Anhui Agricultural Science Bulletin;2009-01
2 ZHANG Bi-xian et al(College of Life Sciences,Northeast Agricultural University,Harbin,Heilongjiang 150030);Research Progress of Plant Chalcone Synthase(CHS) and Its Gene[J];Journal of Anhui Agricultural Sciences;2012-20
3 LI Li~1 SUN Xin~(1,2) MA Jun-lan~1 ZHAO Yue~1 (1.Northeast Agricultural University,Harbin 150030;2.Soybean Research Institute of Heilongjiang Academy of Agricultural Sciences,Harbin 150086);PROGRESS ON KEY ENZYMES CHS、CHI OF ISOFLAVONES SYNTHESIZE[J];Soybean Science;2007-05
4 REN Lei,WANG Yan,ZHOU Lin,PENG Zhen-hua(Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Research Institute of Forestry,CAF,Beijing 100091,China);Cloning and Expression of PsAP1 Gene Related to Flowering in Tree Peony[J];Acta Botanica Boreali-Occidentalia Sinica;2011-09
5 Xiang Dianjun 1,2 Yin Kuide 3 Man Lili 2 Chen Wenfu 1 Zhao Minghui 1 Xu Zhengjin 1 1 Rice Institution of Shenyang Agricultural University / Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture / Key Laboratory of Northern Japonica Rice Breeding of Liaoning, Shenyang, 110161; 2 Heilongjiang Agricultural Economy Professional College, Mudanjiang, 157041; 3 School of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, 163319;Cloning and Expression Analysis of Cold Stress Transcription Factor BcICE1 from Brassica pekinensis[J];Molecular Plant Breeding;2011-03
6 WANG Yun,CHEN Sheng-yong,LI Guan-kang,HE Ai-ru,YU Xiao-li (Zhanjiang Institute of Agricultural Sciences,Zhanjiang 524094,China);Advances in research on drought resistance in sweetpotato[J];Guangdong Agricultural Sciences;2011-11
7 QI Lizhi SHENG Ran YAN Sumei* ZHAO Yanli (College of Animal Science,Inner Mongolia Agricultural University,Huhhot 010018,China);Effects of Acetate Concentration on Triacylglycerol Content and Expression Levels of Lept in and PPARγ Genes in Bovine Mammary Epithelial Cells[J];Chinese Journal of Animal Nutrition;2013-07
8 WANG Xiumei1 HOU Xianzhi1* AO Changjin1 GAO Min2 KAO Guilan1 GAO Aiwu3 LAN Rubing1 TA Na1 (1.College of Animal Science and Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China; 2.Inner Mongolia Academy of Agriculture and Animal Husbandry,Hohhot 010010,China;3.College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China);Effects of Incubation Time on Casein Gene Expressions in Bovine Mammary Epithelial Cells in Three-Dimensional Culture[J];Chinese Journal of Animal Nutrition;2013-07
9 ZHANG Dongmei1 HOU Xianzhi1 YANG Jinli1* GAO Aiwu2 WANG Hairong1 KAO Guilan3(1.College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China;2.College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China;3.College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China);Dietary Restriction and Realimentation on Energy and Nitrogen: Effects on Liver Weight,Proliferation and Expansion of Liver Cells and Expression Levels of GHR and IGFs Genes in Liver of Mongolian Lambs[J];Chinese Journal of Animal Nutrition;2013-07
10 NI Juan 1,LI Zhen 1,XUE Jing-lun 2,WANG Xu 1* (1. School of Life Sciences, Yunnan Normal University, Engineering Research Center of Sustainable Development and Utilizaton of Biomass Energy, Ministry of Education, Kunming 650500, Yunnan; 2. Institute of Genetics, Fudan University, Shanghai 200433, China);Effects of (-)epigallocatechin gallate on expressions of hMLH1 and hMSH2[J];Carcinogenesis,Teratogenesis & Mutagenesis;2013-04
China Proceedings of conference Full-text Database 10 Hits
1 REN Lei WANG Yan ZHOU Lin PENG Zhen-hua (Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Research Institute of Forestry,CAF,Beijing100091);Isolation and Expression Analysis of SEPALATA Gene in Tree Peony[A];[C];2011
2 ;植物查尔酮合成酶分子生物学研究进展及展望[A];[C];2006
3 ZHOU Lin WANG Yan PENG Zhen-hua (Key Laboratory of Tree Breeding and Biotechnology of State Forestry Administration, Research Institute of Forestry,CAF,Beijing 100091);Isolation and Tissue-specific Expression Analysis of Chalcone Synthase Gene Ps-CHS1 in Tree Peony[A];[C];2010
4 Haixia Zhao~(a#),Ying Li~(a#),Yuzhong Wang~(b#),Jing Zhang~c,Xiaoming Ouyang~a, Renxiu Peng~a,Jing Yang~(a*) (a Department of Pharmacology,School of Medicine,Wuhan University,Wuhan 430071,China;b Key Laboratory for Oral Biomedical Engineering of Ministry of Education,School and Hospital of Stomatology,Wuhan University,Wuhan 430079, China;c Animal Experimental Center of Wuhan University,Wuhan 430071,China);Antitumor and immunostimulatory activity of a polysaccharide-protein complex from Scolopendra subspinipes mutilans L.Koch in tumor-bearing mice[A];[C];2013
5 Zhigang Wang,Zhi Yan,Bicheng Zhang,Zhiguo Rao,Lifang Yu,Jian Liu, Jianfei Gao 1Department of Oncology,2 Department of Digestive Diseases,Wuhan General Hospital of Guangzhou Command,People's Liberation Army,Wuhan 430070,P.R. China.;Biomarkers rediscovery using random forests approach based on microarray data of colon cancer[A];[C];2012
6 Ruiwen Fan Shanshan Yang Zhanquan Shi Kaiyuan Ji Xiaoyun Jia Jianbo Yao George W Smith Changsheng Dong~*;Identification of a novel miRNA important for melanogenesis in alpaca(Lama Pacos)[A];[C];2013
7 Huang Qiang Gong Yuehua Wei Anqing Peng Xiaoling (The 94~(th) hospital of PLA Jiangxi Nanchang 330002);Effects of hBD-3 on Staphylococcus aureus biofilm formation-regulating genes dltB and icaA genes[A];[C];2013
8 ZHANG Chao WANG Yan-jie WANG Wei-ning DONG Li (College of Landscape Architecture,Beijing Forestry University,National Flower Engineering Technology Research Center,Beijing 100083);Anthocyanins Accumulation and Expression of Related Genes Involved in Anthocyanin Biosynthesis in the Blotch and Non-blotch Parts of Tree Peony 'Luoyang Hong'[A];[C];2013
9 ;Functional analysis of 30K proteins during silk gland degeneration by a caspase-dependent pathway in Bombyx[A];[C];2013
10 ZHANG Li-qun;HE Xing-han;MALIYA Qiqige;HASI Surong;College of Veterinary Medicine,Inner Mongolia Agricultural University;Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease,Ministry of Agriculture;Veterinary Bureau of Wushen;;Detection of CYP2J Gene Expression in 8 Different Tissues of Bactrian Camel by Using Real-time Quantitative PCR[A];[C];2014
Chinese Journal Full-text Database 10 Hits
1 PENG Shuai et al(Institute of Life Science and Biotechnology,Bioenergy and Biomaterial Research Center,Hunan University,Changsha,Hunan 410082);Analysis of Genetic Relationship of 17 Houttuynia cordate Germplasm Resources With ISSR Marker[J];Journal of Anhui Agricultural Sciences;2007-12
2 LU Hong-Mei1,GUO Fang-Qiu1,WU Xian-Jin 2,LIANG Yi-Zeng11(Research Center for Modernization of Chinese Herbal Medicine,College of Chemistry and Chemical Engineering,Central South University,Changsha 410083)2(Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources,Department of Life Science,Huaihua College,Huaihua 418008);Analysis of Related Volatile Constituents in Houttuynia Cordata Injection and Its Raw Material Herb[J];Chinese Journal of Analytical Chemistry;2011-11
3 XU Xiang-qian;XU Qian-lei;WU Xing-wei;XIE Shi-ping;LI Zhen;GUO Hui-jun;XU Li-ran;FU Lin-chun;Henan Province Hospital of TCM;The First Ailiated Hospital of Henan University of TCM;AIDS Study Institute of Henan University of TCM;Guangzhou University of Chinese Medicine;Henan Key Laboratory of Viral Diseases Control with TCM;;Status and thoughts of research in therapeutic effect evaluation and benefits of TCM prevention and treatment of HIV/AIDS[J];China Journal of Traditional Chinese Medicine and Pharmacy;2013-12
4 TAN Yun;Department of Life Science and Technology,Central South University of Forestry & Technology;;Application of RAPD Molecular Marker Technology in Plant Research[J];Journal of Anhui Agricultural Sciences;2013-25
5 CHEN Qiang(Fujian Zhongte Pharmaceutical Limited Company,Fuzhou,350012;China);Study on GC Fingerprint of Jinghua weikang Capsule[J];Strait Pharmaceutical Journal;2005-01
6 LIU Wen-long,ZHANG Xi-ling,NIE Zhi-jiao,HUANG Sheng,ZOU Huan,YAN Zi-wei(TCM University of Hunan,Changsha,Hunan 410208,China);Study of cordate houttuynia on variation of genetic polymorphism fingerprinting[J];Journal of Traditional Chinese Medicine University of Hunan;2010-07
7 LIU Wen-long1,2,ZHANG Xi-li2,HE Fu-yuan2,WANG Hai-qin2,JIANG XIng-ming2, SHI Ji-lian2,YANG Yan-tao2,BAO Xiao-yan2,CHEN Zuo-hong1(1.College of Life and Science,Hunan Normal University,Changsha 410081,China; 2.College of Pharmacy,TCM University of Hunan,Changsha 410208,China);Study on genetic polymorphism between sampes of Houttuynia herb from same GAP area[J];Journal of Traditional Chinese Medicine University of Hunan;2011-11
8 YANG Li-min,ZHANG Yong-gang,LIN Hong-mei,HAN Zhong-ming,YANG LiCollege of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China;Research Advances on Formation Theories and Control Techniques of Chinese Medicinal Materials Quality[J];Journal of Jilin Agricultural University;2012-02
9 ZHANG Xi-li1,LIU Wen-long1,2,3,HE Fu-yuan1,3,WANG Hai-qin1,YANG Yan-tao1,3,SHI Ji-lian1,3,LI Shun-xiang1,4*(1.Key Laboratory of Hunan Chinese Medicine modernization,School of Pharmacy,Hunan University of CM,Changsha 410208,China;2.College of Life and Science,Hunan Normal University,Changsha 410081,China;3.Key Lab of TCMM,State Administration of TCM,Changsha 410208,China;4.Key Laboratory of Research and Exploiture of CM,CM Academy of Hunan,Changsha,Hunan 410013,China);Study on the differences of total protein contents in different strains of fresh Houttuynia cordata[J];Journal of Traditional Chinese Medicine University of Hunan;2013-05
10 JI Yu-Liang;LI Dui-Shu;ZHAO Long-Fei;WEI Ge-Hong;Biological and Medical Engineering Department,Shangluo University;College of Life Sciences,Shangqiu Normal University;Northwest Agriculture and Forestry University,College of Life Sciences;;Genetic Diversity and Phylogeny of Rhizobia Isolated from Robinia pseudoacacia in Several Regions of Shaanxi Shangluo[J];Journal of Agricultural Biotechnology;2013-11
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 MIAO Jun,HUO Yu-meng,YANG Yan-yan,XIU Jing-run,LIU Bing-jiang,ZHANG Yi-hui,HUO Feng-mei,WU Xiong(Institute of Vegetables,Shandong Academy of Agricultural Sciences,Jinan 250100,China);Improvement of hiTAIL-PCR and Its Application in Onion[J];Shandong Agricultural Sciences;2009-10
2 ZHAO Wei et al (College of Life Science and Technology,University of Electronic Science and Technology,Chengdu,Sichuan 610054);An Effective Method of DNA Extraction from Crassulaceae Plant[J];Journal of Anhui Agricultural Sciences;2006-22
3 XIA Hui-ling et al(Jiangxi-OAI Joint Research Institute,Nanchang University,Nanchang,Jiangxi 330047);Extraction and Purification of Dihydromyricetin in Ampelopsis grossedentata[J];Journal of Anhui Agricultural Sciences;2011-32
4 LIAO Jingjun\ AN Chengcai\ WU Si\ CHEN Zhangliang (The National Laboratory of protein Engineering and Plant Genetic Engineering,College of Life Sciences,Peking University,Beijing,100871);The Role of Chalcone Synthase Gene in the Defense Response of Higher Plants against Pathogens[J];ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS;2000-04
5 HAN Ying-ying, MING Feng, WANG Jing-wen, LIANG Bin, GUO Bin, YE Ming-ming, SHEN Da-leng (Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, State Key Laboratory of Genetic Engineering,Institute of Genetics, School of Life Sciences, Fudan University,Shanghai 200433, China);The Cloning and Identification of Chalcone Synthase Gene cDNA of Phalaenopsis and Its Expression in E.coli[J];Journal of Fudan University;2004-02
6 XU Feng1,2,CHENG Shui-yuan1,3*,WANG Yan1,LI Lin-ling1,CHENG Shu-han2(1College of Horticulture and Gardening,Yangtze University,Jingzhou,Hubei 434025 China;2College of Horticulture Science and Engineering,Shandong Agricultural University,Taian,Shandong 271018 China;3College of Life Science and Engineering,Huanggang Normal University,Huanggang,Hubei 438000 China);Efficient amplification and sequence analysis of chalcone synthase gene from Ginkgo biloba by thermal asymmetric interlaced PCR[J];Journal of Fruit Science;2007-02
7 Zheng Cen 1,2 Zhang Liping 1,2 Tang Zhonghui 2 Zhao Changping1,2 Yuan Shaohua 2 1 College of Life Science, Capital Normal University, Beijing, 100048;2 Beijing Engineering Research Center for Hybrid Wheat, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097;TAIL-PCR and its Cloning in Plant Gene[J];Genomics and Applied Biology;2009-03
8 XIONG Pu1, YAO Mao-jun2, XIAO Kai-jun1 (1.College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641) (2.College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China);Extraction of Dihydromyricetin from Ampelopsis[J];Modern Food Science and Technology;2009-08
9 WANG Yan,LIU Xiao-nian,GU Zhen-hua,ZHU Fa-ren,CHEN Chang-yuan(Changde Vocational Technical College,Changde 415000,China);Research Progress of Ornamental Peach-blossom in China[J];Journal of Hebei Agricultural Sciences;2008-06
10 YI Cheng(Vocational Technique of Environment and Biology College of Hunan, Hengyang 421005, Hunan,China);Research Development of Ampelopsis grossedentata[J];Economic Forest Researches;2004-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved